Abstract：
Taxus contorta (family Taxaceae) is a native plant of temperate region of western Himalaya. The current study investigated the effect of altitude on the phytochemical composition and mycorrhizal diversity, associated with distribution of T. contorta in Shimla district, Himachal Pradesh, India. Quantitative phytochemical analysis of the leaf extracts indicated that alkaloid levels decreased with altitude, with the highest value in Himri\'s methanol extracts (72.79 ± 1.08 mg/g) while phenol content increased with altitude, peaking in Nankhari\'s methanol extracts (118.83 ± 5.90 mg/g). Saponin content was higher in methanol extracts (78.13 ± 1.66 mg/g in Nankhari, 68.06 ± 1.92 mg/g in Pabbas, and 56.32 ± 1.93 mg/g in Himri). Flavonoid levels were notably higher in chloroform extracts, particularly in Nankhari (219.97 ± 2.99 mg/g), and positively correlated with altitude. Terpenoids were higher in chloroform extracts at Himri (11.34 ± 0.10 mg/g) and decreased with altitude. Taxol content showed minimal variation between solvents and altitudes (4.53-6.98 ppm), while rutin was only detected in methanol extracts (1.31-1.46 ppm). Mycorrhizal spore counts in T. contorta\'s rhizosphere varied with altitude: highest at Himri (77.83 ± 2.20 spores/50 g soil), decreasing to Pabbas (68.06 ± 1.96 spores/50 g soil) and lowest at Nankhari (66.00 ± 2.77 spores/50 g soil), with 17 AMF species identified overall, showing significant altitudinal influence on spore density. The rhizosphere of T. contorta was shown to be dominated by the Glomus species. The rhizospheric soil of the plant was found to be slightly acidic. Organic carbon and available potassium content decreased contrasting with increasing available nitrogen and phosphorus with altitude. Correlation data showed strong negative links between organic carbon (-0.83), moderate positive for nitrogen (0.46) and phosphorus (0.414), and moderate negative for potassium (-0.56) with the altitude. This study provides a comprehensive insight into changes in phytochemical constituents, mycorrhizal diversity and soil composition of T. contorta along a range of altitude.
摘要：
紫杉（紫杉科）是喜马拉雅西部温带地区的本地植物。本研究调查了海拔高度对植物化学成分和菌根多样性的影响，与西姆拉地区的T.contorta分布有关，喜马al尔邦,印度。叶提取物的定量植物化学分析表明，生物碱水平随海拔高度而降低，在Himri的甲醇提取物中具有最高的值（72.79±1.08mg/g），而苯酚含量随海拔高度而增加，在Nankhari的甲醇提取物中达到峰值（118.83±5.90mg/g）。甲醇提取物中皂苷含量较高(南喀里78.13±1.66mg/g,68.06±1.92毫克/克在帕巴，和56.32±1.93毫克/克在Himri)。氯仿提取物中的类黄酮含量明显较高，特别是在南喀里（219.97±2.99毫克/克），与海拔呈正相关。Himri时氯仿提取物中萜类化合物含量较高（11.34±0.10mg/g），并随海拔高度而降低。紫杉醇含量显示出溶剂和海拔高度之间的最小变化（4.53-6.98ppm），而芦丁仅在甲醇提取物中检测到（1.31-1.46ppm）。T.contorta根际的菌根孢子计数随海拔高度而变化：在Himri最高（77.83±2.20孢子/50g土壤），减少到Pabbas（68.06±1.96孢子/50克土壤），最低的是Nankhari（66.00±2.77孢子/50克土壤），总共确定了17个AMF物种，显示出显着的海拔对孢子密度的影响。T.contorta的根际被证明是由Glomus物种主导的。发现植物的根际土壤呈微酸性。有机碳和速效钾含量随海拔高度的增加而降低。相关数据显示有机碳(-0.83)，氮(0.46)和磷(0.414)中度阳性，和中度负钾（-0.56）与海拔高度。这项研究为植物化学成分的变化提供了全面的见解，沿一定海拔高度的T.contorta的菌根多样性和土壤组成。
