Abstract：
The effects of important nutrients such as calcium (Ca), copper (Cu), iron (Fe), magnesium (Mg), selenium (Se), and zinc (Zn) have been investigated in relation to male fertility due to their roles in proper spermatogenesis, sperm maturation, motility, and optimal sperm function. An imbalance between these elements has been associated with several pathologic conditions and male reproductive issues. The purpose of this study was to determine the essential trace and electrolytes elements, such as Ca, Cu, Fe, Mg, Se, and Zn, in human biological samples (blood, serum, and semen) from patients with male infertility. This study used correlational analysis to determine the potential associations between these elements and male fertility. Imbalances in these elements have been linked to various pathological conditions and male reproductive issues. One hundred eighty referent male adults and two hundred twenty-nine patients diagnosed with subtypes of infertility were included in the study, divided into two age groups. Acid digestion was controlled using a microwave oven, and the essential trace elements and electrolytes in the oxidized biological samples were determined using atomic absorption spectrometry. Certified reference materials of blood and serum were used to validate the accuracy of the methodology. The results showed that the concentrations of Ca, Cu, Fe, Mg, Se, and Zn in the blood, serum, and seminal plasma of male adults in all age groups were higher than those in patients with different infertility phenotypes. Essential element deficiency in all biological fluid samples may significantly negatively affect human reproductive health and lead to male infertility. Through a multidimensional approach, our study sought to unravel the intricate biochemical signatures associated with OAT, providing insights that may shape the landscape of diagnostic and therapeutic strategies for male reproductive health.
摘要：
钙（Ca）等重要营养素的影响，铜(Cu),铁(Fe),镁(Mg),硒(Se),和锌（Zn）由于它们在适当的精子发生中的作用而与男性生育能力有关，精子成熟,运动性,和最佳的精子功能。这些元素之间的不平衡与几种病理状况和男性生殖问题有关。这项研究的目的是确定必需的痕量和电解质元素，比如Ca,Cu,Fe,Mg,Se,Zn,在人类生物样本(血液，血清,和精液）来自男性不育患者。这项研究使用相关分析来确定这些元素与男性生育力之间的潜在关联。这些要素的失衡与各种病理状况和男性生殖问题有关。这项研究包括了一百八十名男性成年人和二百二十九名被诊断患有不孕症亚型的患者，分为两个年龄组。用微波炉控制酸消化，用原子吸收光谱法测定氧化生物样品中必需的微量元素和电解质。使用经过认证的血液和血清参考材料来验证该方法的准确性。结果表明,Ca的浓度,Cu,Fe,Mg,Se,血液中的锌，血清,各年龄组男性成人精浆均高于不同表型不育患者。所有生物体液样本中的必需元素缺乏可能会严重影响人类生殖健康并导致男性不育。通过多维方法，我们的研究试图解开与OAT相关的复杂生化特征，提供可能塑造男性生殖健康诊断和治疗策略的见解。
