PDF(Pubmed)
Abstract：
Functional M cells are differentiated by receptor activator of NF-κB ligand (RANKL) and capture of luminal antigens to initiate immune responses. We aimed to use postbiotic-based recombinant chicken RANKL (cRANKL) to promote M cell differentiation and test the efficacy of oral vaccines. Chicks were divided into three groups that were administered phosphate-buffered saline (PBS), cell extracts of wild-type Lactococcus lactis subsp. lactis IL1403 (WT_CE), or cell extracts of recombinant L. lactis expressing cRANKL (cRANKL_CE). The expression of the M cell marker was measured, and the gut microbiome was profiled. The efficiency of the infectious bursal disease (IBD) vaccine was tested after 12 consecutive days of administering cRANKL_CE. The chickens that were administered cRANKL_CE (p = 0.038) had significantly higher Annexin A5 (ANXA5) mRNA expression levels than those in the PBS group (PBS vs. WT_CE, p = 0.657). In the gut microbiome analysis, no significant changes were observed. However, the relative abundance of Escherichia-Shigella was negatively correlated (r = - 0.43, p = 0.019) with ANXA5 mRNA expression in Peyer\'s patches. cRANKL_CE/IBD (p = 0.018) had significantly higher IBD-specific faecal IgA levels than PBS/IBD (PBS/IBD vs. WT_CE/IBD, p = 0.217). Postbiotic-based recombinant cRANKL effectively improved the expression of M cell markers and the efficiency of oral vaccines. No significant changes were observed in the gut microbiome after administration of postbiotic-based recombinant cRANKL. This strategy can be used for the development of feed additives and adjuvants. KEY POINTS: • Postbiotic-based recombinant cRANKL enhanced the expression of ANXA5 in chicken. • The relative abundance of Escherichia-Shigella was negatively correlated with ANXA5 expression. • Postbiotic-based recombinant cRANKL effectively improved the efficiency of oral vaccine.
摘要：
功能性M细胞通过NF-κB配体（RANKL）的受体激活剂分化，并捕获腔抗原以启动免疫应答。我们旨在使用基于后生物的重组鸡RANKL（cRANKL）促进M细胞分化并测试口服疫苗的功效。小鸡被分为三组，分别给予磷酸盐缓冲盐水（PBS），野生型乳酸乳球菌亚种的细胞提取物。乳酸IL1403(WT_CE)，或表达cRANKL(cRANKL_CE)的重组乳酸乳球菌的细胞提取物。测量M细胞标志物的表达，并对肠道微生物组进行了分析。在连续12天施用cRANKL_CE后测试传染性法氏囊病(IBD)疫苗的效率。给予cRANKL_CE（p=0.038）的鸡的膜联蛋白A5（ANXA5）mRNA表达水平明显高于PBS组（PBSvs.WT_CE,p=0.657)。在肠道微生物组分析中，没有观察到显著的变化。然而,大肠杆菌-志贺氏菌的相对丰度与Peyer\'s斑块中ANXA5mRNA表达呈负相关（r=-0.43，p=0.019）。cRANKL_CE/IBD（p=0.018）的IBD特异性粪便IgA水平明显高于PBS/IBD（PBS/IBD与WT_CE/IBD，p=0.217）。基于后生物的重组cRANKL有效地提高了M细胞标志物的表达和口服疫苗的效率。在施用基于后生物的重组cRANKL后,在肠道微生物组中没有观察到显著变化。该策略可用于饲料添加剂和佐剂的开发。关键点:•基于后生生物的重组cRANKL增强ANXA5在鸡中的表达。•大肠杆菌志贺氏菌的相对丰度与ANXA5表达呈负相关。•基于后生物的重组cRANKL有效地提高了口服疫苗的效率。
