PDF(Pubmed)
Abstract：
The continuously evolving PRRSV has been plaguing pig farms worldwide for over 30 years, with conventional vaccines suffering from insufficient protection and biosecurity risks. To address these challenges, we identified 10 PRRSV-specific CTL epitopes through enzyme-linked immunospot assay (ELISPOT) and constructed a multi-epitope peptide (PTE) by linking them in tandem. This PTE was then fused with a modified porcine Fc molecule to create the recombinant protein pFc-PTE. Our findings indicate that pFc-PTE effectively stimulates PRRSV-infected specific splenic lymphocytes to secrete high levels of interferon-gamma (IFN-γ) and is predicted to be non-toxic and non-allergenic. Compared to PTE alone, pFc-PTE not only induced a comparable cellular immune response in mice but also extended the duration of the immune response to at least 10 weeks post-immunization. Additionally, pFc-PTE predominantly induced a Th1 immune response, suggesting its potential advantage in enhancing cellular immunity. Consequently, pFc-PTE holds promise as a novel, safe, and potent candidate vaccine for PRRSV and may also provide new perspectives for vaccine design against other viral diseases.
摘要：
不断发展的PRRSV已经困扰着全球养猪场30多年，传统疫苗存在保护不足和生物安全风险。为了应对这些挑战，我们通过酶联免疫斑点试验(ELISPOT)鉴定了10个PRRSV特异性CTL表位,并通过串联连接构建了多表位肽(PTE).然后将该PTE与修饰的猪Fc分子融合以产生重组蛋白pFc-PTE。我们的发现表明，pFc-PTE有效刺激PRRSV感染的特异性脾淋巴细胞分泌高水平的干扰素-γ（IFN-γ），并被预测为无毒和非过敏性。与单独的PTE相比，pFc-PTE不仅在小鼠中诱导相当的细胞免疫应答,而且将免疫应答的持续时间延长至免疫后至少10周。此外,pFc-PTE主要诱导Th1免疫应答，表明其在增强细胞免疫方面的潜在优势。因此,pFc-PTE作为小说有希望，安全,和有效的PRRSV候选疫苗，也可能为针对其他病毒性疾病的疫苗设计提供新的视角。
