PDF(Pubmed)
Abstract：
Ischemic teat necrosis (ITN) is a growing problem in the dairy industry characterized by teat lesions, necrosis, pruritus and automutilation. Despite the economic and welfare consequences, there is no treatment, and the etiology of the disease remains poorly understood. The aim of this study was to investigate ITN by analyzing its clinical presentation, potential risk factors and microbial involvement. Methods included collection of milk and swab samples from affected cows over a period of one-and-a-half years and completion of questionnaires by veterinarians and farmers. Microbial testing included PCR testing for Treponema spp. and cultural testing by anaerobic and aerobic incubation on blood agar. The results showed a high and significant prevalence of Treponema spp. and Staphylococcus aureus in affected teats compared to non-ITN-affected control teats, indicating their potential role in the development of ITN. Other factors such as edema and milking practices also appear to contribute to the tissue damage. First-lactation and early-lactation heifers are particularly at risk. In conclusion, ITN appears to have a multifactorial etiology with both infectious and non-infectious factors playing a role. Further research is needed to better understand the complex interplay of these factors and to develop effective prevention and management strategies.
摘要：
缺血性乳头坏死（ITN）是乳品行业中日益严重的问题，其特征是乳头病变。坏死,瘙痒和自动化。尽管经济和福利后果，没有治疗,和疾病的病因仍然知之甚少。这项研究的目的是通过分析其临床表现来研究ITN，潜在的危险因素和微生物参与。方法包括在一年半的时间内从受影响的奶牛中收集牛奶和拭子样本，并由兽医和农民完成问卷调查。微生物检测包括密螺旋体的PCR检测。以及通过在血琼脂上厌氧和有氧培养进行培养测试。结果表明，密螺旋体属的患病率高且显着。和金黄色葡萄球菌在受影响的奶头与非受ITN影响的对照奶头相比，表明它们在ITN发展中的潜在作用。其他因素如水肿和挤奶行为似乎也有助于组织损伤。首次泌乳和早期泌乳的小母牛尤其处于危险之中。总之,ITN似乎具有多因素病因，感染和非感染因素均起作用。需要进一步研究，以更好地了解这些因素之间复杂的相互作用，并制定有效的预防和管理策略。
