PDF(Pubmed)
Abstract：
Platelet-derived growth factor B (PDGFB), as an important cellular growth factor, is widely involved in the regulation of cellular events such as cell growth, proliferation, and differentiation. Although important, the expression characteristics and biological functions in the mammalian reproductive system remain poorly understood. In this study, the PDGFB gene of Tibetan sheep was cloned by RT-PCR, and its molecular characteristics were analyzed. Subsequently, the expression of the PDGFB gene in the testes and epididymides (caput, corpus, and cauda) of Tibetan sheep at different developmental stages (3 months, 1 year, and 3 years) was examined by qRT-PCR and immunofluorescence staining. A bioinformatic analysis of the cloned sequences revealed that the CDS region of the Tibetan sheep PDGFB gene is 726 bp in length and encodes 241 amino acids with high homology to other mammals, particularly goats and antelopes. With the increase in age, PDGFB expression showed an overall trend of first decreasing and then increasing in the testis and epididymis tissues of Tibetan sheep, and the PDGFB mRNA expression at 3 months of age was extremely significantly higher than that at 1 and 3 years of age (p < 0.05). The PDGFB protein is mainly distributed in testicular red blood cells and Leydig cells in Tibetan sheep at all stages of development, as well as red blood cells in the blood vessel, principal cells, and the pseudostratified columnar ciliated epithelial cells of each epididymal duct epithelium. In addition, PDGFB protein expression was also detected in the spermatocytes of the 3-month-old group, spermatids of the 1-year-old group, spermatozoa and interstitial cells of the 3-year-old group, and loose connective tissue in the epididymal duct space in each developmental period. The above results suggest that the PDGFB gene, as an evolutionarily conserved gene, may play multiple roles in the development and functional maintenance of testicular cells (such as red blood cells, Leydig cells, and germ cells) and epididymal cells (such as red blood cells, principal cells, and ciliated epithelial cells) during testicular and epididymal development, which lays a foundation for the further exploration of the mechanisms by which the PDGFB gene influences spermatogenesis in Tibetan sheep.
摘要：
血小板源性生长因子B(PDGFB),作为一种重要的细胞生长因子，广泛参与细胞生长等细胞事件的调节，扩散,和差异化。虽然重要,哺乳动物生殖系统的表达特征和生物学功能仍然知之甚少。在这项研究中,用RT-PCR方法克隆了藏羊的PDGFB基因,并对其分子特征进行了分析。随后，PDGFB基因在睾丸和附睾中的表达(caput，语料库，和cauda）在不同发育阶段（3个月，1年,和3年）通过qRT-PCR和免疫荧光染色进行检查。对克隆序列进行生物信息学分析,发现藏羊PDGFB基因CDS区长726bp,编码241个氨基酸,与其他哺乳动物具有较高的同源性,尤其是山羊和羚羊。随着年龄的增长,PDGFB在藏羊睾丸和附睾组织中的表达总体上呈现先降低后升高的趋势,3月龄PDGFBmRNA表达极显著高于1、3月龄(p<0.05)。PDGFB蛋白主要分布在藏羊各个发育阶段的睾丸红细胞和睾丸间质细胞中,以及血管中的红细胞，主要细胞,和每个附睾管上皮的假复层柱状纤毛上皮细胞。此外,3月龄组精母细胞中也检测到PDGFB蛋白表达，1岁组的精子细胞，3岁组的精子和间质细胞，和每个发育期附睾导管间隙的疏松结缔组织。上述结果表明,PDGFB基因,作为进化上保守的基因，可能在睾丸细胞（如红细胞，Leydig细胞,和生殖细胞）和附睾细胞（如红细胞，主要细胞,和纤毛上皮细胞）在睾丸和附睾发育期间，为进一步探索PDGFB基因影响藏绵羊精子发生的机制奠定了基础。
