PDF(Pubmed)
Abstract：
There is a growing interest in the composition of amniotic fluid (AF) in both humans and animals. In addition to its nutritional and protective functions for the foetus, current knowledge demonstrates that AF also serves advanced diagnostic, prognostic, and therapeutic roles. Newborn dogs have an underdeveloped immune system, making them highly susceptible to dangerous pathogens such as canine parvovirus (CPV-2), canine infectious hepatitis virus (CAdV-1), and canine distemper virus (CDV), thus exposing them to a high risk of mortality in the first weeks of life. Immunoglobulins G (IgGs) represent the only antibody isotype capable of crossing the placenta in a small amount and have been detected also in canine AF. The primary aim of this study was to investigate the reliability of AF collected at birth as a marker of passive immunity in canine species. For this purpose, total and specific IgGs against CPV-2, CAdV-1, and CDV were investigated and quantified in both maternal plasma and AF collected at the time of caesarean section. The vaccination status of the bitches was also taken into consideration. Since the immune system can be influenced by gestational age, with preterm infants having immature innate and adaptive immunity, IgG concentrations were correlated with amniotic lecithin, sphingomyelin, cortisol, surfactant protein A, and pentraxin 3 levels. In a previous study from our group on foetal maturity these molecules were measured in the same samples. Finally, correlations between their amniotic content and neonatal outcomes were investigated. This study demonstrates that AF analysis at birth can provide valuable insights into neonatal immunity in puppies, offering a non-invasive method to detect potential early health risks, for improved puppy care and management.
摘要：
人类和动物对羊水（AF）的组成越来越感兴趣。除了它对胎儿的营养和保护功能外，目前的知识表明，AF也提供先进的诊断，预后，和治疗作用。新生狗的免疫系统不发达,使它们对危险的病原体高度敏感，如犬细小病毒（CPV-2），犬传染性肝炎病毒（CAdV-1），和犬瘟热病毒（CDV），从而使他们在生命的最初几周面临很高的死亡风险。免疫球蛋白G（IgG）代表能够少量穿过胎盘的唯一抗体同种型，并且在犬AF中也已检测到。这项研究的主要目的是研究出生时收集的AF作为犬科动物被动免疫标志物的可靠性。为此,在剖腹产时收集的母体血浆和AF中,对针对CPV-2,CAdv-1和CDV的总IgG和特异性IgG进行了调查和定量.还考虑了母狗的疫苗接种状况。由于免疫系统会受到胎龄的影响，早产儿有不成熟的先天和适应性免疫，IgG浓度与羊水卵磷脂相关，鞘磷脂,皮质醇,表面活性剂蛋白A,和pentraxin3级。在我们小组先前关于胎儿成熟度的研究中，在相同的样品中测量了这些分子。最后,调查了羊膜含量与新生儿结局之间的相关性。这项研究表明，出生时的AF分析可以为幼犬的新生儿免疫提供有价值的见解，提供一种非侵入性方法来检测潜在的早期健康风险，改善小狗的护理和管理。
