PDF(Pubmed)
Abstract：
Bovine coronavirus (BCoV), bovine rotavirus, bovine viral diarrhea virus, and bovine astrovirus are the most common intestinal pathogenic viruses causing diarrhea in cattle. We collected 1646 bovine fecal samples from January 2020 to August 2023. BCoV was the major pathogen detected, with a positive rate of 34.02% (560/1646). Of the 670 diarrheal samples and 976 asymptomatic samples, 209 and 351 were BCoV-positive, respectively. Studying the relevance of diarrhea associated with BCoV has shown that the onset of diarrheal symptoms post-infection is strongly correlated with the cattle\'s age and may also be related to the breed. We amplified and sequenced the hemagglutinin esterase (HE), spike protein, and whole genomes of the partially positive samples and obtained six complete HE sequences, seven complete spike sequences, and six whole genomes. Molecular characterization revealed that six strains were branched Chinese strains, Japanese strains, and partial American strains from the GⅡb subgroup. Strains HBSJZ2202 and JSYZ2209 had four amino acid insertions on HE. We also analyzed ORF1a and found disparities across various regions within GIIb, which were positioned on separate branches within the phylogenetic tree. This work provides data for further investigating the epidemiology of BCoV and for understanding and analyzing BCoV distribution and dynamics.
摘要：
牛冠状病毒（BCoV），牛轮状病毒,牛病毒性腹泻病毒,牛星状病毒是引起牛腹泻的最常见的肠道病原病毒。我们从2020年1月至2023年8月收集了1646份牛粪便样本。BCoV是检测到的主要病原体，阳性率为34.02%(560/1646)。在670份腹泻样本和976份无症状样本中，209和351为BCoV阳性，分别。研究与BCoV相关的腹泻的相关性表明，感染后腹泻症状的发作与牛的年龄密切相关，也可能与品种有关。我们扩增并测序了血凝素酯酶（HE），刺突蛋白，和部分阳性样本的整个基因组，并获得了六个完整的HE序列，七个完整的尖峰序列，和六个完整的基因组。分子鉴定表明，有6个菌株是分枝的中国菌株，日本菌株，和来自GⅡb亚组的部分美国菌株。菌株HBSJZ2202和JSYZ2209在HE上有四个氨基酸插入。我们还分析了ORF1a，发现GIIb中各个地区的差异，它们位于系统发育树中的不同分支上。这项工作为进一步调查BCoV的流行病学以及了解和分析BCoV的分布和动态提供了数据。
