PDF(Pubmed)
Abstract：
Chrysin is hypothesized to possess the ability to prevent different illnesses, such as diabetes, cancer, and neurodegenerative disorders. Nonetheless, chrysin has a low solubility under physiological conditions, resulting in limited bioavailability. In a previous study, we utilized an oil-in-water emulsion system (chrysin-ES or chrysin-NE) to encapsulate chrysin, thereby increasing its bioaccessibility and preserving its antioxidant and anti-Alzheimer\'s properties. To promote the chrysin-ES as a supplementary and functional food, it was obligatory to carry out a safety assessment. Cytotoxicity testing showed that chrysin-ES was harmless, with no killing effect on 3T3-L1 (adipocytes), RAW 264.7 (macrophages), HEK293 (kidney cells), and LX-2 (hepatic stellate cells). The acute toxicity evaluation demonstrated that the 50% lethal dose (LD50) for chrysin-ES was greater than 2000 mg/kg BW. Genotoxicity assessments found that chrysin-ES did not induce DNA mutations in vitro or in vivo. Furthermore, chrysin and chrysin-ES exhibited anti-mutagenic properties against PhIP-induced and IQ-induced mutagenesis in the Ames test, while they inhibited urethane-, ethyl methanesulfonate-, mitomycin C-, and N-nitrosomethylurea-mediated mutations in Drosophila. The present study illustrates the safety and anti-genotoxicity properties of chrysin-ES, allowing for the further development of chrysin-based food supplements and nutraceuticals.
摘要：
假设Chyrin具有预防不同疾病的能力，比如糖尿病，癌症,和神经退行性疾病。尽管如此，chrysin在生理条件下溶解度低，导致有限的生物利用度。在之前的研究中,我们利用水包油乳液系统（chrysin-ES或chrysin-NE）来封装chrysin，从而增加其生物可及性和保持其抗氧化和抗阿尔茨海默病的性质。为了推广chrysin-ES作为辅助和功能食品，必须进行安全性评估。细胞毒性试验表明chrysin-ES是无害的,对3T3-L1（脂肪细胞）没有杀伤作用，RAW264.7（巨噬细胞），HEK293（肾细胞），和LX-2（肝星状细胞）。急性毒性评估表明，chrysin-ES的50％致死剂量（LD50）大于2000mg/kgBW。遗传毒性评估发现，chrysin-ES在体外或体内均未诱导DNA突变。此外，在Ames试验中，chrysin和chrysin-ES对PhIP诱导和IQ诱导的诱变表现出抗诱变特性，虽然他们抑制了氨基甲酸酯-，甲磺酸乙酯-，丝裂霉素C-,和果蝇中N-亚硝基甲基脲介导的突变。本研究说明了chrysin-ES的安全性和抗遗传毒性特性，允许进一步开发基于chrysin的食品补充剂和营养保健品。
