PDF(Pubmed)
Abstract：
Telomerase is an enzyme involved in the maintenance of telomeres. Telomere shortening due to the end-replication problem is a threat to the genome integrity of all eukaryotes. Telomerase inside cells depends on a myriad of protein-protein and RNA-protein interactions to properly assemble and regulate the function of the telomerase holoenzyme. These interactions are well studied in model eukaryotes, like humans, yeast, and the ciliated protozoan known as Tetrahymena thermophila. Emerging evidence also suggests that deep-branching eukaryotes, such as the parasitic protist Trypanosoma brucei require conserved and novel RNA-binding proteins for the assembly and function of their telomerase. In this review, we will discuss telomerase regulatory pathways in the context of telomerase-interacting proteins, with special attention paid to RNA-binding proteins. We will discuss these interactors on an evolutionary scale, from parasitic protists to humans, to provide a broader perspective on the extensive role that protein-protein and RNA-protein interactions play in regulating telomerase activity in eukaryotes.
摘要：
端粒酶是一种参与端粒维持的酶。由于末端复制问题导致的端粒缩短对所有真核生物的基因组完整性构成威胁。细胞内的端粒酶依赖于无数的蛋白质-蛋白质和RNA-蛋白质相互作用来正确组装和调节端粒酶全酶的功能。这些相互作用在模型真核生物中得到了很好的研究，像人类一样，酵母，和纤毛原生动物被称为嗜热四膜虫。新出现的证据还表明，深分支真核生物，例如寄生原生的布鲁氏锥虫需要保守的和新的RNA结合蛋白来组装和发挥其端粒酶的功能。在这次审查中,我们将在端粒酶相互作用蛋白的背景下讨论端粒酶调节途径，特别关注RNA结合蛋白。我们将在进化的尺度上讨论这些相互作用者，从寄生原生生物到人类，为蛋白质-蛋白质和RNA-蛋白质相互作用在调节真核生物端粒酶活性中的广泛作用提供更广泛的视角。
