PDF(Pubmed)
Abstract：
Timely and accurate detection and characterization of microbial threats is crucial for effective infection and outbreak management. Additionally, in food production, rapid microbe identification is indispensable for maintaining quality control and hygiene standards. Current methods for typing microbial strains often rely on labor-intensive, time-consuming, and expensive DNA- and sera-serotyping techniques, limiting their applicability in rapid-response scenarios. In this context, the IR Biotyper®, utilizing Fourier-transform infrared (FTIR) spectroscopy, offers a novel approach, providing specific spectra for fast strain typing within 3 h. This methodology article serves as a comprehensive resource for researchers and technicians aiming to utilize FTIR spectroscopy for microbial strain typing. It encompasses detailed guidelines on sample preparation, data acquisition, and analysis techniques, ensuring the generation of reliable and reproducible results. We highlight the IR Biotyper®\'s rapid and accurate discrimination capabilities, showcasing its potential for real-time pathogen monitoring and source-tracking to enhance public health and food safety. We propose its integration as an early screening method, followed by more detailed analysis with whole-genome sequencing, to optimize detection accuracy and response efficiency in microbial surveillance systems.
摘要：
及时准确地检测和表征微生物威胁对于有效的感染和爆发管理至关重要。此外,在食品生产中，快速微生物识别对于维持质量控制和卫生标准是必不可少的。目前的微生物菌株分型方法通常依赖于劳动密集型,耗时,和昂贵的DNA和血清分型技术，限制了它们在快速反应场景中的适用性。在这种情况下，IRBiotyper®，利用傅里叶变换红外（FTIR）光谱，提供了一种新颖的方法，为3小时内的快速菌株分型提供特定的光谱。本方法文章作为研究人员和技术人员的综合资源，旨在利用FTIR光谱进行微生物菌株分型。它包括关于样品制备的详细指南，数据采集,和分析技术，确保产生可靠和可重复的结果。我们强调IRBiotyper®的快速和准确的辨别能力，展示了其实时病原体监测和源头跟踪以增强公共卫生和食品安全的潜力。我们建议将其整合作为一种早期筛查方法，接下来是更详细的全基因组测序分析，优化微生物监测系统的检测精度和响应效率。
