PDF(Pubmed)
Abstract：
Characterising inner ear disorders represents a significant challenge due to a lack of reliable experimental procedures and identified biomarkers. It is also difficult to access the complex microenvironments of the inner ear and investigate specific pathological indicators through conventional techniques. Omics technologies have the potential to play a vital role in revolutionising the diagnosis of ear disorders by providing a comprehensive understanding of biological systems at various molecular levels. These approaches reveal valuable information about biomolecular signatures within the cochlear tissue or fluids such as the perilymphatic and endolymphatic fluid. Proteomics identifies changes in protein abundance, while metabolomics explores metabolic products and pathways, aiding the characterisation and early diagnosis of diseases. Although there are different methods for identifying and quantifying biomolecules, mass spectrometry, as part of proteomics and metabolomics analysis, could be utilised as an effective instrument for understanding different inner ear disorders. This study aims to review the literature on the application of proteomic and metabolomic approaches by specifically focusing on Meniere\'s disease, ototoxicity, noise-induced hearing loss, and vestibular schwannoma. Determining potential protein and metabolite biomarkers may be helpful for the diagnosis and treatment of inner ear problems.
摘要：
由于缺乏可靠的实验程序和鉴定的生物标志物,表征内耳疾病代表了重大挑战。通过常规技术也很难进入内耳的复杂微环境并研究特定的病理指标。通过在各种分子水平上提供对生物系统的全面了解，组学技术有可能在革命性的耳部疾病诊断中发挥至关重要的作用。这些方法揭示了关于耳蜗组织或流体(诸如外淋巴和内淋巴流体)内的生物分子特征的有价值的信息。蛋白质组学识别蛋白质丰度的变化，代谢组学探索代谢产物和途径，帮助疾病的表征和早期诊断。虽然有不同的方法来识别和量化生物分子，质谱,作为蛋白质组学和代谢组学分析的一部分，可以用作了解不同内耳疾病的有效工具。本研究旨在通过特别关注梅尼埃病的蛋白质组学和代谢组学方法的应用综述文献。耳毒性,噪声引起的听力损失，前庭神经鞘瘤.确定潜在的蛋白质和代谢物生物标志物可能有助于内耳问题的诊断和治疗。
