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Abstract：
Haemonchus contortus, a blood-feeding parasite in grazing sheep, causes economic losses. Drug resistance necessitates exploring plant-based anthelmintics like Artemisia cina (Asteraceae). The plant, particularly its ethyl acetate extract, shows anthelmintic activity against H. contortus. However, there is limited information on pharmacodynamic interactions in ethyl acetate compounds. The study aims to identify pharmacodynamic interactions in the ethyl acetate extract of A. cina with anthelmintic effects on H. contortus eggs and L3 larvae using binary mixtures. Bioactive compounds were isolated via chromatography and identified using spectroscopic techniques. Pharmacodynamic interactions were assessed through binary mixtures with a main compound. Four bioactive compounds were identified: 1-nonacosanol, hentriacontane, peruvin, and cinic acid. Binary mixtures, with peruvin as the main compound, were performed. Peruvin/1-nonacosanol-hentriacontane and peruvin/cinic acid mixtures demonstrated 1.42-fold and 4.87-fold increased lethal effects in H. contortus L3 infective larvae, respectively, at a 0.50LC25/0.50LC25 concentration. In this work, we determined the synergism between bioactive compounds isolated from the ethyl acetate extract of A. cina and identified unreported compounds for the specie.
摘要：
Haemonchuscontrortus，放牧绵羊的吸血寄生虫，造成经济损失。耐药性需要探索基于植物的驱虫药，例如青蒿（菊科）。植物,特别是它的乙酸乙酯提取物，显示了对H.contortus的驱虫活性。然而,关于乙酸乙酯化合物的药效学相互作用的信息有限。该研究旨在使用二元混合物确定A.cina的乙酸乙酯提取物中的药效学相互作用，对H.contortus卵和L3幼虫具有驱虫作用。通过色谱法分离生物活性化合物并使用光谱技术进行鉴定。通过与主要化合物的二元混合物评估药效学相互作用。确定了四种生物活性化合物：1-壬烷醇，Hentriacontane,Peruvin,还有肉桂酸.二元混合物,以Peruvin为主要化合物，被执行了。Peruvin/1-nonacosol-hentriacontane和peruvin/cinicacid混合物在H.contortusL3感染幼虫中的致死作用增加了1.42倍和4.87倍，分别,在0.50LC25/0.50LC25浓度。在这项工作中,我们确定了从A.cina的乙酸乙酯提取物中分离出的生物活性化合物之间的协同作用，并确定了该物种的未报道化合物。
