PDF(Pubmed)
Abstract：
Babesiosis is a growing concern due to the increased prevalence of this infectious disease caused by Babesia protozoan parasites, affecting various animals and humans. With rising worries over medication side effects and emerging drug resistance, there is a notable shift towards researching babesiacidal agents. Antimicrobial peptides, specifically cathelicidins known for their broad-spectrum activity and immunomodulatory functions, have emerged as potential candidates. Aquiluscidin, a cathelicidin from Crotalus aquilus, and its derivative Vcn-23, have been of interest due to their previously observed antibacterial effects and non-hemolytic activity. This work aimed to characterize the effect of these peptides against three Babesia species. Results showed Aquiluscidin\'s significant antimicrobial effects on Babesia species, reducing the B. bigemina growth rate and exhibiting IC50 values of 14.48 and 20.70 μM against B. ovata and B. bovis, respectively. However, its efficacy was impacted by serum presence in culture, and it showed no inhibition against a B. bovis strain grown in serum-supplemented medium. Conversely, Vcn-23 did not demonstrate babesiacidal activity. In conclusion, Aquiluscidin shows antibabesia activity in vitro and its efficacy is affected by the presence of serum in the culture medium. Nevertheless, this peptide represents a candidate for further investigation of its antiparasitic properties and provides insights into potential alternatives for the treatment of babesiosis.
摘要：
由于巴贝斯虫原生动物寄生虫引起的这种传染病的患病率增加，巴贝斯虫病日益受到关注，影响各种动物和人类。随着对药物副作用和新出现的耐药性的担忧日益增加，有一个明显的转变，研究婴儿的药物。抗菌肽,特别是以广谱活性和免疫调节功能而闻名的cathelicidins，已经成为潜在的候选人。Aquiluscidin,来自Crotalusaquilus的cathelicidin，及其衍生物Vcn-23由于其先前观察到的抗菌作用和非溶血活性而受到关注。这项工作旨在表征这些肽对三种巴贝虫物种的作用。结果表明，Aquiluscidin对Babesia物种具有显着的抗菌作用，降低Bigemina的生长速率，对卵黄芽孢杆菌和牛芽孢杆菌的IC50值分别为14.48和20.70μM，分别。然而,其功效受培养物中血清存在的影响，并且它显示出对在补充血清的培养基中生长的牛芽孢杆菌菌株没有抑制作用。相反，Vcn-23没有表现出杀虫活性。总之,Aquiluscidin在体外显示出抗babesia活性，其功效受培养基中血清存在的影响。然而，该肽代表了进一步研究其抗寄生虫特性的候选物，并为治疗巴贝斯虫病的潜在替代方法提供了见解。
