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Abstract：
Balantioides coli is a ciliated protist that can cause dysentery in humans, pigs and nonhuman primates and may have the potential for zoonotic transmission. Its diagnosis is routinely performed through conventional parasitological techniques, and few studies have used culturing techniques to isolate it, applying molecular tools for the characterization of this protozoan. Thus, the objective of this study was to confirm B. coli diagnosis using molecular tools and to characterize the genetic variants of this parasite isolated from pigs kept on family farms in Brazil using three different culture media that differed in the serum added. Fecal samples from pigs were inoculated in Pavlova medium plus coconut water (PC), fetal bovine serum (PB) and horse serum (PH). Of the 127 samples positive for forms compatible with the phylum Ciliophora, 31 were selected for isolation. The most successful medium for isolation was PB 19/31 (61.3%), followed by PH 18/31 (58.1%) and PC 11/31 (35.5%). Of the nucleotide sequences generated, 20 were classified as genetic variant type B0, two as A1 and 15 as A0. The results indicated that PC, despite having allowed the isolation of B. coli for a short period, was not an adequate medium for the maintenance of this parasite in vitro, therefore requiring improvement.
摘要：
大肠杆菌Balantioides是一种有纤毛的原生生物，可以引起人类痢疾，猪和非人灵长类动物，可能具有人畜共患传播的潜力。它的诊断通常通过常规的寄生虫学技术进行，很少有研究使用培养技术来分离它，应用分子工具来表征这种原生动物。因此，这项研究的目的是使用分子工具确认大肠杆菌的诊断，并使用三种不同的培养基来表征从巴西家庭农场饲养的猪中分离出的这种寄生虫的遗传变异。将猪的粪便样品接种在Pavlova培养基加椰子水（PC）中，胎牛血清(PB)和马血清(PH)。在127个与纤毛门相容的样品中，选择31个进行分离。最成功的分离培养基是PB19/31（61.3％），其次是PH18/31（58.1%）和PC11/31（35.5%）。在产生的核苷酸序列中，20个分为遗传变异型B0，两个分为A1和15个分为A0。结果表明,PC,尽管允许在短时间内分离大肠杆菌，不是维持这种寄生虫在体外的适当培养基，因此需要改进。
