PDF(Pubmed)
Abstract：
The Chikungunya virus (CHIKV) presents global health challenges, with Brazil experiencing outbreaks since its introduction in 2014. In 2023, following a CHIKV outbreak in Minas Gerais (MG), social media was used to optimize an entomological survey aimed at identifying vectors and viral lineages and assessing insecticide resistance. Following Instagram posts, residents with suspected CHIKV infection were able to schedule mosquito aspirations. In total, 421 mosquitoes (165 Aedes aegypti and 256 Culex quinquefasciatus) were captured from 40 households in Salinas city (MG) and tested for the Dengue, Zika, and Chikungunya viruses through RT-qPCR. Twelve of 57 pools (10 Ae. aegypti and two Cx. quinquefasciatus) tested positive for CHIKV RNA. Viral RNA was also detected in the heads of nine Ae. aegypti, indicating viral dissemination but not in Cx. quinquefasciatus. Genome sequencing yielded the first near-complete genome from the 2023 outbreak, unveiling that the CHIKV strain belonged to the East/Central/South African (ECSA) genotype. Additionally, genetic analyses revealed high frequencies of kdr alleles, including in CHIKV-infected mosquitoes, suggesting resistance to pyrethroid insecticides in this Ae. aegypti population. Social media was important for guiding mosquito-capture efforts in CHIKV transmission hotspots, thus optimizing the opportunity for viral detection. These findings emphasize the urgent need for innovative vector studies and control strategies, as well as interdisciplinary approaches in public health interventions.
摘要：
基孔肯雅病毒（CHIKV）带来了全球健康挑战，巴西自2014年引入以来经历了疫情。2023年，在米纳斯吉拉斯州(MG)发生CHIKV疫情后，社交媒体用于优化昆虫学调查，旨在识别载体和病毒谱系并评估杀虫剂抗性。在Instagram帖子之后，怀疑有CHIKV感染的居民能够安排蚊子的愿望。总的来说,从萨利纳斯市（MG）的40户家庭中捕获了421只蚊子（165只埃及伊蚊和256只库蚊），并进行了登革热测试，Zika,和基孔肯雅病毒通过RT-qPCR。57个游泳池中的12个（10Ae。埃及伊蚊和两个CX。quinquefasciatus）检测为CHIKVRNA阳性。在9个Ae的头部也检测到病毒RNA。埃及伊蚊,表明病毒传播，但不在CX。Quinquefasciatus.基因组测序产生了2023年爆发的第一个近乎完整的基因组，宣布CHIKV菌株属于东/中/南非（ECSA）基因型。此外,遗传分析显示kdr等位基因的频率很高，包括感染CHIKV的蚊子，表明该Ae对拟除虫菊酯杀虫剂具有抗性。埃及伊蚊种群。社交媒体对于指导CHIKV传播热点地区的蚊子捕获工作非常重要，从而优化病毒检测的机会。这些发现强调迫切需要创新的媒介研究和控制策略，以及公共卫生干预中的跨学科方法。
