PDF(Pubmed)
Abstract：
The occurrence of antibiotic-resistant bacteria in foodstuff involves a human health risk. Edible insects are a precious resource; however, their consumption raises food safety issues. In this study, the occurrence of antibiotic resistant bacteria in laboratory-reared fresh mealworm larvae (Tenebrio molitor L.) and frass was assessed. Antibiotics were not used during the rearing. Enterobacteriaceae and enterococci were isolated from 17 larvae and eight frass samples. In total, 62 and 69 isolates presumed to belong to Enterobacteriaceae and Enterococcus spp., respectively, were obtained and tested for antibiotic susceptibility via disk diffusion. Based on the results, isolates were grouped, and representative resistant isolates were identified at species level through 16S rRNA gene sequencing. For enterococci resistance, percentages higher than 15% were observed for vancomycin and quinupristin-dalfopristin, whereas Enterobacteriaceae resistance higher than 25% was found against cefoxitin, ampicillin, and amoxicillin-clavulanic acid. Based on the species identification, the observed resistances seemed to be intrinsic both for enterococci and Enterobacteriaceae, except for some β-lactams resistance in Shigella boydii (cefoxitin and aztreonam). These could be due to transferable genetic elements. This study suggests the need for further investigations to clarify the role of edible insects in the spreading of antibiotic resistance determinants through the food chain.
摘要：
食品中抗生素抗性细菌的出现涉及人类健康风险。食用昆虫是一种宝贵的资源；然而，他们的消费引发了食品安全问题。在这项研究中,评估了实验室饲养的新鲜粉虫幼虫（TenebriomolitorL.）和frass中抗生素抗性细菌的发生。在饲养期间不使用抗生素。从17个幼虫和8个frass样品中分离出肠杆菌科和肠球菌。总的来说,推测属于肠杆菌科和肠球菌属的62和69个分离株。,分别,获得并通过圆盘扩散测试抗生素敏感性。根据结果,分离株被分组，并通过16SrRNA基因测序在物种水平鉴定了代表性抗性分离株。对于肠球菌耐药性，万古霉素和喹诺普汀-达福普汀的百分比高于15%，而肠杆菌科细菌对头孢西丁的抗性高于25%，氨苄青霉素,和阿莫西林-克拉维酸.根据物种鉴定，观察到的耐药性似乎对肠球菌和肠杆菌科都是内在的，除了一些β-内酰胺类耐药的志贺氏菌（头孢西丁和氨曲南）。这些可能是由于可转移的遗传元件。这项研究表明，有必要进行进一步的研究，以阐明食用昆虫在通过食物链传播抗生素抗性决定因素中的作用。
