PDF(Pubmed)
Abstract：
Effective root canal disinfection and the subsequent release of natural growth factors from dentin are crucial to the success of regenerative endodontic procedures. This study evaluated the effect of newly introduced calcium silicate-based temporary intracanal medicament Bio-C Temp and calcium hydroxide-based material UltraCal XS on the release of transforming growth factor β1 (TGF-β1) from root canal dentin. Twenty-two intact and fully developed human premolars from patients aged 15-18 were shaped and irrigated according to the current clinical recommendations. The teeth were then gently split in half, and the root canal dentin of paired samples was covered with Bio-C Temp or UltraCal XS. After 3 weeks of incubation, the specimens were conditioned with 17% EDTA and the collected solution was subjected to the quantification of the released TGF-β1 by performing an ELISA. One-way analysis of variance (ANOVA), followed by Tukey\'s test, was selected to determine the statistically significant differences between the groups at the 0.95 confidence level. The highest mean value of released TGF-β1 (1993.1 pg/mL) was detected in the control group, where the root canal dentin was conditioned with 17% EDTA alone. Regarding the experimental groups, Bio-C Temp released a statistically significantly higher amount of TGF-β1 (282.14 pg/mL) compared to UltraCal XS (114.28 pg/mL; p = 0.0158). Bio-C Temp affected the release of growth factors from root canal dentin less than UltraCal XS and may therefore serve as an intracanal medicament for regenerative endodontic procedures.
摘要：
有效的根管消毒和随后从牙本质中释放自然生长因子对于再生牙髓手术的成功至关重要。这项研究评估了新引入的基于硅酸钙的临时管内药物Bio-CTemp和基于氢氧化钙的材料UltraCalXS对根管牙本质中转化生长因子β1（TGF-β1）释放的影响。根据当前的临床建议，对来自15-18岁患者的22个完整且完全发育的人类前磨牙进行了整形和灌溉。然后牙齿被轻轻地分成两半，并用Bio-CTemp或UltraCalXS覆盖配对样品的根管牙本质。经过3周的孵化，用17%EDTA调节样本,并通过进行ELISA对收集的溶液进行释放的TGF-β1的定量。单因素方差分析(ANOVA)接下来是Tukey的测试，选择以确定在0.95置信水平下组间的统计学显著差异。对照组中TGF-β1释放值最高(1993.1pg/mL),其中根管牙本质用17％EDTA单独调节。关于实验组，与UltraCalXS（114.28pg/mL；p=0.0158）相比，Bio-CTemp释放的TGF-β1（282.14pg/mL）具有统计学意义。Bio-CTemp对根管牙本质中生长因子的释放影响小于UltraCalXS，因此可以用作再生牙髓手术的肛门内药物。
