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Abstract：
Microscopic colitis (MC) is classified as collagenous colitis (CC) and lymphocytic colitis (LC). Genetic associations between CC and human leucocyte antigens (HLAs) have been found, with smoking being a predisposing external factor. Smoking has a great impact on metabolomics. The aim of this explorative study was to analyze global metabolomics in MC and to examine whether the metabolomic profile differed regarding the type and course of MC, the presence of IBS-like symptoms, treatment, and smoking habits. Of the 240 identified women with MC aged ≤73 years, 131 completed the study questionnaire; the Rome III questionnaire; and the Visual Analog Scale for Irritable Bowel Syndrome (VAS-IBS). Blood samples were analyzed by ultra-high-performance liquid chromatograph mass spectrometry (UHLC-MS/UHPLC-MSMS). The women, 63.1 (58.7-67.2) years old, were categorized based on CC (n = 76) and LC (n = 55); one episode or refractory MC; IBS-like symptoms or not; use of corticosteroids or not; and smoking habits. The only metabolomic differences found in the univariate model after adjustment for false discovery rate (FDR) were between smokers and non-smokers. Serotonin was markedly increased in smokers (p < 0.001). No clear patterns appeared when conducting a principal component analysis (PCA). No differences in the metabolomic profile were found depending on the type or clinical course of the disease, neither in the whole MC group nor in the subgroup analysis of CC.
摘要：
显微镜结肠炎(MC)分为胶原性结肠炎(CC)和淋巴细胞性结肠炎(LC)。已经发现CC和人类白细胞抗原（HLAs）之间的遗传关联，吸烟是一个诱发外部因素。吸烟对代谢组学有很大影响。这项探索性研究的目的是分析MC中的全球代谢组学，并检查代谢组学概况是否在MC的类型和过程中有所不同。IBS样症状的存在，治疗,和吸烟习惯。在240名MC年龄≤73岁的女性中，131完成了研究问卷；罗马III问卷；以及肠易激综合征视觉模拟量表（VAS-IBS）。通过超高效液相色谱质谱(UHLC-MS/UHPLC-MSMS)分析血液样品。女人，63.1（58.7-67.2）岁，根据CC(n=76)和LC(n=55)进行分类;一次发作或难治性MC;是否出现IBS样症状;是否使用皮质类固醇;和吸烟习惯.在校正错误发现率（FDR）后，在单变量模型中发现的唯一代谢组学差异在吸烟者和非吸烟者之间。5-羟色胺在吸烟者中显著增加(p<0.001)。进行主成分分析（PCA）时没有出现清晰的模式。根据疾病的类型或临床病程，未发现代谢组学谱的差异，无论是在整个MC组还是在CC的亚组分析中。
