PDF(Pubmed)
Abstract：
Despite the central role of cats in the transmission and amplification of Sporothrix, studies regarding immune response in feline sporotrichosis are scarce. In cats with sporotrichosis, neutrophil-rich lesions are usually associated to good general condition and lower fungal burden. However, the role of neutrophils in anti-Sporothrix immunity has been little explored in cats. Thus, the aim of this study was to evaluate the neutrophil oxidative burst in the blood of cats with sporotrichosis. Cats with sporotrichosis included in the study were treated with itraconazole (ITZ) alone or combined with potassium iodide (KI). The neutrophil oxidative burst was evaluated through a flow-cytometry-based assay using dihydrorhodamine 123 (background) and stimulation with Zymosan and heat-killed Sporothrix yeasts. The cure rate was 50.0% in cats under treatment with ITZ monotherapy and 90.9% in cats treated with ITZ + KI (p = 0.014), endorsing the combination therapy as an excellent alternative for the treatment of feline sporotrichosis. Higher percentages of Sporothrix-stimulated neutrophils were associated with good general condition (p = 0.003). Higher percentages of Sporothrix- (p = 0.05) and Zymosan-activated (p = 0.014) neutrophils before and early in the treatment were related to clinical cure in ITZ-treated cats. The correlation between oxidative burst and successful use of KI could not be properly assessed given the low number of failures (n = 2) in this treatment group. Nasal mucosa involvement, typically linked to treatment failure, was related to lower percentages of activated neutrophils in the background at the treatment outcome (p = 0.02). Our results suggest a beneficial role of neutrophils in feline sporotrichosis and a positive correlation between neutrophil activation and the cure process in ITZ-treated cats.
摘要：
尽管猫在孢子丝菌的传播和扩增中起着核心作用，关于猫孢子丝菌病免疫反应的研究很少。在孢子丝菌病的猫中，富含中性粒细胞的病变通常与良好的一般状况和较低的真菌负担相关。然而,中性粒细胞在抗孢子丝菌免疫中的作用在猫中的研究很少。因此，这项研究的目的是评估孢子丝菌病猫血液中中性粒细胞的氧化爆发。研究中包括的孢子丝菌病猫单独用伊曲康唑（ITZ）或与碘化钾（KI）联合治疗。通过使用二氢罗丹明123（背景）的基于流式细胞术的测定以及用酵母聚糖和热杀死的孢子丝菌酵母的刺激来评估中性粒细胞氧化爆发。在用ITZ单药治疗的猫中治愈率为50.0％，在用ITZKI治疗的猫中治愈率为90.9％（p=0.014），支持联合疗法作为治疗猫孢子丝菌病的极好替代方法。较高百分比的孢子丝菌刺激的嗜中性粒细胞与良好的一般状况相关(p=0.003)。在ITZ治疗的猫中，在治疗之前和早期，孢子丝菌（p=0.05）和酵母聚糖激活（p=0.014）的嗜中性粒细胞的较高百分比与临床治愈有关。考虑到该治疗组中失败次数少（n=2），无法正确评估氧化爆发与KI成功使用之间的相关性。鼻粘膜受累，通常与治疗失败有关，与治疗结果时背景中活化中性粒细胞百分比较低有关(p=0.02)。我们的结果表明，中性粒细胞在猫孢子丝菌病中的有益作用以及中性粒细胞活化与ITZ治疗猫的治愈过程之间的正相关。
