PDF(Pubmed)
Abstract：
The incidence of Candida infections has increased in the last decade, posing a serious threat to public health. Appropriately facing this challenge requires precise epidemiological data on species and antimicrobial resistance incidence, but many countries lack appropriate surveillance programs. This study aims to bridge this gap for Morocco by identifying and phenotyping a year-long collection of clinical isolates (n = 93) from four clinics in Tetouan. We compared the current standard in species identification with molecular methods and assessed susceptibility to fluconazole and anidulafungin. Our results identified limitations in currently used diagnostics approaches, and revealed that C. albicans ranks as the most prevalent species with 60 strains (64.52%), followed by C. glabrata with 14 (15.05%), C. parapsilosis with 6 (6.45%), and C. tropicalis with 4 (4.30%). In addition, we report the first identification of C. metapsilosis in Morocco. Susceptibility results for fluconazole revealed that some isolates were approaching MICs resistance breakpoints in C. albicans (2), and C. glabrata (1). Our study also identified anidulafungin resistant strains in C. albicans (1), C. tropicalis (1), and C. krusei (2), rendering the two strains from the latter species multidrug-resistant due to their innate resistance to fluconazole. These results raise concerns about species identification and antifungal resistance in Morocco and highlight the urgent need for more accurate methods and preventive strategies to combat fungal infections in the country.
摘要：
念珠菌感染的发病率在过去十年有所增加，对公众健康构成严重威胁。适当面对这一挑战需要关于物种和抗菌素耐药性发生率的准确流行病学数据，但许多国家缺乏适当的监测方案。这项研究旨在通过从Tetouan的四个诊所中鉴定和表型收集长达一年的临床分离株（n=93）来弥合摩洛哥的这一差距。我们将目前的物种鉴定标准与分子方法进行了比较，并评估了对氟康唑和anidulafungin的敏感性。我们的结果确定了目前使用的诊断方法的局限性，并显示白色念珠菌是最流行的物种，有60株(64.52%)，其次是光滑梭菌，占14(15.05%)，C.近apsilia与6（6.45％），和热带梭状芽胞杆菌4（4.30％）。此外,我们报告了摩洛哥首次鉴定的C.对氟康唑的敏感性结果表明，一些分离株正在接近白色念珠菌的MIC抗性断点（2），和C.glabrata(1)。我们的研究还确定了白色念珠菌中的抗anidulafungin菌株(1)，C.热带（1），和C.krusei(2)，由于对氟康唑的先天抗性，使后者的两种菌株具有多重耐药性。这些结果引起了人们对摩洛哥物种鉴定和抗真菌耐药性的关注，并强调迫切需要更准确的方法和预防策略来对抗该国的真菌感染。
