PDF(Pubmed)
Abstract：
The genome of Trametes versicolor encodes multiple laccase isozymes, the expression of which is responsive to various conditions. Here, we set out to investigate the potential of orange peel extract as an inducer of laccase production in this white-rot fungus, in comparison to the previously identified inducing chemical compound, veratryl alcohol. For four geographically distinct T. versicolor strains, a positive correlation has been observed between their oxidative activity and incubation time in liquid cultures. The addition of 20% orange peel extract or 5 mM veratryl alcohol caused a rapid increase in the oxidative potential of T. versicolor M99 after 24 h, with a more pronounced effect observed for the orange peel extract. To elucidate the underlying molecular mechanisms of the induced laccase activity, a transcriptional gene expression analysis was performed for the seven individual laccase genes in T. versicolor, revealing the upregulation of several laccase genes in response to the addition of each inducer. Notably, the gene encoding TvLac5 demonstrated a substantial upregulation in response to the addition of 20% orange peel extract, likely contributing to the observed increase in its oxidative potential. In conclusion, our results demonstrate that orange peels are a promising agro-industrial side stream for implementation as inducing agents in large-scale laccase production with T. versicolor.
摘要：
杂色Trametes的基因组编码多种漆酶同工酶，其表达响应于各种条件。这里,我们着手研究橙皮提取物作为这种白腐真菌中漆酶生产诱导剂的潜力，与先前确定的诱导化合物相比，veratryl醇.对于四个地理上不同的杂色T.sicolor菌株，在液体培养物中，它们的氧化活性与孵育时间之间存在正相关。添加20％的橘皮提取物或5mM藜芦醇导致24小时后杂色T.M99的氧化电位迅速增加，对于橘皮提取物观察到更明显的效果。为了阐明诱导漆酶活性的潜在分子机制，对杂色毛虫中的七个单独的漆酶基因进行了转录基因表达分析，揭示了响应于每种诱导物的添加，几种漆酶基因的上调。值得注意的是,编码TvLac5的基因在添加20%橙皮提取物时表现出显著的上调，可能有助于观察到其氧化潜能的增加。总之,我们的结果表明，橙皮是一种有前途的农业工业侧流，可作为诱导剂在用花色T.
