PDF(Pubmed)
Abstract：
Biocolourants could be a sustainable option for dyes that require fossil-based chemicals in their synthesis. We studied the in vitro toxicity of anthraquinone aglycone extract obtained from Cortinarius sanguineus fungus and compared it to the toxicity of its two main components, emodin and previously studied dermocybin. Cell viability, cytotoxicity, and oxidative stress responses in HepG2 liver and THP-1 immune cell lines were studied along with skin sensitisation. In addition, genotoxicity was studied with comet assay in HepG2 cells. Cellular viability was determined by MTT, propidium iodide, and lactate dehydrogenase assays, which showed that the highest doses of both the aglycone extract and emodin affected the viability. However, the effect did not occur in all of the used assays. Notably, after both exposures, a dose-dependent increase in oxidative stress factors was observed in both cell lines as measured by MitoSOX and dihydroethidium assays. C. sanguineus extract was not genotoxic in the comet assay. Importantly, both emodin and the extract activated the skin sensitisation pathway in the KeratinoSens assay, suggesting that they can induce allergy in humans. As emodin has shown cytotoxic and skin-sensitising effects, it is possible that the adverse effects caused by the extract are also mediated by it since it is the main component present in the fungus.
摘要：
对于在合成过程中需要化石基化学物质的染料，生物着色剂可能是一种可持续的选择。我们研究了从sanginois真菌中获得的蒽醌苷元提取物的体外毒性，并将其与其两种主要成分的毒性进行了比较，大黄素和以前研究过的皮糖素。细胞活力，细胞毒性，研究了HepG2肝脏和THP-1免疫细胞系中的氧化应激反应以及皮肤致敏。此外,用彗星实验研究了HepG2细胞的遗传毒性。通过MTT测定细胞活力，碘化丙啶,和乳酸脱氢酶测定，这表明糖苷配基提取物和大黄素的最高剂量都会影响生存力。然而,在所有使用的试验中均未出现该效应.值得注意的是,在两次曝光之后，通过MitoSOX和二氢乙啶测定，在两种细胞系中均观察到氧化应激因子的剂量依赖性增加。血梭菌提取物在彗星试验中没有遗传毒性。重要的是,大黄素和提取物在KeratinoSens试验中激活了皮肤致敏途径，表明它们可以在人类中引起过敏。大黄素已显示出细胞毒性和皮肤致敏作用，由于提取物是真菌中存在的主要成分，因此提取物引起的副作用也可能是由其介导的。
