PDF(Pubmed)
Abstract：
Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is one of the most devastating agricultural pests worldwide due to its high reproductive and invasive abilities. The elucidation of its gonadal developmental characteristics and the identification of sex-related genes will provide a useful genetic basis for reproductive-based pest control. Here, the gonadal transcriptome of B. dorsalis was sequenced, and novel gonad-specific expressed genes were analyzed. A total of 1338, 336, 35, and 479 differentially expressed genes (DEGs) were found in the testis (TE), ovary (OV), female accessory gland (FAG), and male accessory gland (MAG), respectively. Furthermore, 463 highly expressed gonad-specific genes were identified, with the TE having the highest number of specific highly expressed genes, at 402, followed by 51 in the OV, 9 in the MAG, and only 1 in the FAG. Strikingly, approximately half of highly expressed gonad-specific genes were uncharacterized. Then, it was found that 35, 17, 3, 2, and 1 of 202 uncharacterized highly expressed TE-specific genes encoded proteins that contained transmembrane domains, signal peptides, high-mobility group boxes, the zinc finger domain, and the BTB/POZ domain, respectively. Interestingly, approximately 40% of uncharacterized highly expressed gonad-specific genes encoding proteins were not predicted to possess functional motifs or domains. Finally, the spatiotemporal expression and sequence characterization of six novel highly expressed gonad-specific genes were analyzed. Altogether, our findings provide a valuable dataset for future functional analyses of sex-related genes and potential target sites for pest control.
摘要：
dorsalis（Hendel）（双翅目：Tephritidae）是全球最具破坏性的农业害虫之一，因为它具有很高的繁殖和入侵能力。对其性腺发育特征的阐明和性别相关基因的鉴定将为基于生殖的害虫防治提供有用的遗传基础。这里,对背芽孢杆菌的性腺转录组进行了测序，并分析了新的性腺特异性表达基因。在睾丸（TE）中发现了1338、336、35和479个差异表达基因（DEGs），卵巢（OV），女性附件腺（FAG），和男性附件腺（MAG），分别。此外，确定了463个高表达的性腺特异性基因，TE具有最高数量的特定高表达基因，402，其次是OV的51，9在MAG中，FAG中只有1个。引人注目的是，大约一半的高表达性腺特异性基因未表征。然后，发现202个未表征的高表达TE特异性基因中的35、17、3、2和1个编码含有跨膜结构域的蛋白质，信号肽，高流动性组盒，锌指域，和BTB/POZ域，分别。有趣的是,大约40％的未表征的高度表达的性腺特异性基因编码蛋白质没有预测具有功能基序或结构域。最后,分析了六个新的高表达性腺特异性基因的时空表达和序列表征。总之,我们的研究结果为未来性别相关基因的功能分析和害虫防治的潜在靶位点提供了有价值的数据集.
