PDF(Pubmed)
Abstract：
Honeybees (Apis mellifera L.) have to face many challenges, including Varroa destructor infestation, associated with viral transmission. Oxalic acid is one of the most common treatments against Varroa. Little is known about the physiological effects of oxalic acid, especially those on honeybees\' immune systems. In this study, the short-term effects (0-96 h) of oxalic acid treatment on the immune system components (i.e., glucose oxidase, phenoloxidase, glutathione S-transferase, catalase activities, and vitellogenin contents) of house bees were preliminarily investigated. Oxalic acid contents of bee bodies and haemolymphs were also measured. The results confirm that oxalic acid is constitutively present in bee haemolymphs and its concentration is not affected by treatment. At 6 h after the treatment, a maximum peak of oxalic acid content was detected on bees\' bodies, which gradually decreased after that until physiological levels were reached at 48 h. In the immune system, the oxalic acid treatment determined a peak in glucose oxidase activity at 48 h, indicating a potential defence response and an increase in vitellogenin content at 24 h. No significant changes were recorded in phenoloxidase, glutathione S-transferase, and catalase activities. These results suggest a time-dependent response to oxalic acid, with potential immune system activation in treated bees.
摘要：
蜜蜂(ApismelliferaL.)必须面临许多挑战，包括瓦罗亚破坏者侵扰,与病毒传播有关。草酸是针对瓦螨的最常见的治疗方法之一。对草酸的生理作用知之甚少，尤其是那些在蜜蜂免疫系统上。在这项研究中,草酸处理对免疫系统成分的短期影响(0-96小时)(即葡萄糖氧化酶,酚氧化酶,谷胱甘肽S-转移酶，过氧化氢酶活性，和卵黄蛋白原含量）进行了初步调查。还测量了蜂体和血淋巴的草酸含量。结果证实，草酸组成性存在于蜜蜂血淋巴中，其浓度不受治疗影响。在治疗后6小时，在蜜蜂体内检测到草酸含量的最大峰值，之后逐渐下降，直到48小时达到生理水平。在免疫系统中，草酸处理在48小时确定了葡萄糖氧化酶活性的峰值，表明潜在的防御反应和24小时卵黄蛋白原含量的增加。酚氧化酶没有显著变化，谷胱甘肽S-转移酶，和过氧化氢酶活性。这些结果表明对草酸的时间依赖性反应，在处理过的蜜蜂中具有潜在的免疫系统激活。
