PDF(Pubmed)
Abstract：
Agricultural insects play a crucial role in transmitting plant viruses and host a considerable number of insect-specific viruses (ISVs). Among these insects, the white-backed planthoppers (WBPH; Sogatella furcifera, Hemiptera: Delphacidae) are noteworthy rice pests and are responsible for disseminating the southern rice black-streaked dwarf virus (SRBSDV), a significant rice virus. In this study, we analyzed WBPH transcriptome data from public sources and identified three novel viruses. These newly discovered viruses belong to the plant-associated viral family Solemoviridae and were tentatively named Sogatella furcifera solemo-like virus 1-3 (SFSolV1-3). Among them, SFSolV1 exhibited a prevalent existence in different laboratory populations, and its complete genome sequence was obtained using rapid amplification of cDNA ends (RACE) approaches. To investigate the antiviral RNA interference (RNAi) response in WBPH, we conducted an analysis of virus-derived small interfering RNAs (vsiRNAs). The vsiRNAs of SFSolV1 and -2 exhibited typical patterns associated with the host\'s siRNA-mediated antiviral immunity, with a preference for 21- and 22-nt vsiRNAs derived equally from both the sense and antisense genomic strands. Furthermore, we examined SFSolV1 infection and distribution in WBPH, revealing a significantly higher viral load of SFSolV1 in nymphs\' hemolymph compared to other tissues. Additionally, in adult insects, SFSolV1 exhibited higher abundance in male adults than in female adults.
摘要：
农业昆虫在传播植物病毒中起着至关重要的作用，并宿主大量的昆虫特异性病毒（ISV）。在这些昆虫中,白背飞虱（WBPH；Sogatellafurcifera，半翅目：Delphacidae）是值得注意的水稻害虫，并负责传播南方水稻黑条矮缩病毒（SRBSDV），一种重要的水稻病毒。在这项研究中,我们分析了来自公共来源的WBPH转录组数据,并鉴定出3种新病毒.这些新发现的病毒属于植物相关病毒家族Solemoviridae，暂定名为Sogatellafurcifera-3样病毒1-3（SFSolV1-3）。其中,SFSolV1在不同的实验室人群中普遍存在，并使用cDNA末端快速扩增（RACE）方法获得其完整的基因组序列。研究WBPH的抗病毒RNA干扰（RNAi）应答，我们对病毒来源的小干扰RNA(vsiRNAs)进行了分析.SFSolV1和-2的vsiRNAs表现出与宿主的siRNA介导的抗病毒免疫相关的典型模式，优选来自有义和反义基因组链的21-和22-ntvsiRNAs。此外，我们检查了SFSolV1在WBPH中的感染和分布，与其他组织相比，若虫血淋巴中SFSolV1的病毒载量明显更高。此外,在成年昆虫中，SFSolV1在成年男性中的丰度高于成年女性。
