PDF(Pubmed)
Abstract：
In this in vivo study on hairless mice, we examined the effects of light-emitting diode (LED) treatment applied prior to ultraviolet B (UVB) irradiation. We found that pre-treating with LED improved skin morphological and histopathological conditions compared to those only exposed to UVB irradiation. In our study, histological evaluation of collagen and elastic fibers after LED treatment prior to UVB irradiation showed that this pretreatment significantly enhanced the quality of fibers, which were otherwise poor in density and irregularly arranged due to UV exposure alone. This suggests that LED treatment promotes collagen and elastin production, leading to improved skin properties. Additionally, we observed an increase in Claudin-1 expression and a reduction in nuclear factor-erythroid 2-related factor 2 (Nrf-2) and heme-oxygenase 1 (HO-1) expression within the LED-treated skin tissues, suggesting that LED therapy may modulate key skin barrier proteins and oxidative stress markers. These results demonstrate that pretreatment with LED light can enhance the skin\'s resistance to UVB-induced damage by modulating gene regulation associated with skin protection. Further investigations are needed to explore the broader biological effects of LED therapy on other tissues such as blood vessels. This study underscores the potential of LED therapy as a non-invasive approach to enhance skin repair and counteract the effects of photoaging caused by UV exposure.
摘要：
在这项对无毛小鼠的体内研究中，我们检查了在紫外线B（UVB）照射之前应用的发光二极管（LED）处理的效果。我们发现,与仅暴露于UVB照射的那些相比,用LED预处理改善了皮肤形态学和组织病理学状况。在我们的研究中,UVB照射前LED处理后的胶原和弹性纤维的组织学评估表明，这种预处理显着提高了纤维的质量，否则，由于单独的紫外线暴露，密度差并且不规则排列。这表明LED治疗促进胶原蛋白和弹性蛋白的产生，导致改善皮肤性能。此外,我们观察到LED处理的皮肤组织中Claudin-1表达的增加和核因子-红系2相关因子2（Nrf-2）和血红素加氧酶1（HO-1）表达的减少，提示LED治疗可能调节关键的皮肤屏障蛋白和氧化应激标志物。这些结果表明，用LED光预处理可以通过调节与皮肤保护相关的基因调节来增强皮肤对UVB诱导的损伤的抵抗力。需要进一步的研究来探索LED疗法对血管等其他组织的更广泛的生物学效应。这项研究强调了LED疗法作为一种非侵入性方法的潜力，可以增强皮肤修复并抵消紫外线照射引起的光老化的影响。
