PDF(Pubmed)
Abstract：
Extensive research on medicinal herbs for bioactive compounds proposes that they could replace synthetic drugs, reducing side effects and economic burdens. Especially, interest in the synergistic benefits of natural products is increasing, implying that their combined use may enhance therapeutic effectiveness. This study aimed to explore the synergetic effects of turmeric (Curcuma longa L.) and black pepper (Piper nigrum L.) extract on lung normal (MRC-5) and cancer (A549 and NCI-H292) cell lines. The turmeric extract (TM) only affected the lung cancer cell lines, but it had no impact on the MRC-5 cell line. On the other hand, the black pepper extract (BP) did not cause any damage to either the lung normal or cancer cell lines, even at concentrations of up to 400 µg/mL. Response surface methodology was used to predict the ideal synergistic concentrations (EC50) of TM and BP, which were found to be 48.5 and 241.7 µg/mL, respectively. Notably, the selected condition resulted in higher cytotoxicity compared to the exposure to TM alone, indicating a potent synergetic effect. The rate of curcumin degradation under this combined treatment was significantly decreased to 49.72 ± 5.00 nmol/h/µg for A549 cells and 47.53 ± 4.78 nmol/h/µg for NCI-H292 cells, respectively, as compared to curcumin alone. Taken together, this study confirmed the potent synergistic effect of TM and BP on lung cancer cell lines. Further research is required to identify their specific synergetic mechanisms. Our findings provide crucial foundational data on the synergistic effects of TM and BP.
摘要：
对生物活性化合物的草药的广泛研究表明，它们可以替代合成药物，减少副作用和经济负担。尤其是,人们对天然产物的协同作用越来越感兴趣，这意味着它们的联合使用可能会提高治疗效果。本研究旨在探讨姜黄（CurcumalongaL.）和黑胡椒（PipernigrumL.）提取物对肺正常（MRC-5）和癌症（A549和NCI-H292）细胞系的协同作用。姜黄提取物（TM）仅影响肺癌细胞系，但对MRC-5细胞系没有影响。另一方面,黑胡椒提取物（BP）对肺正常或癌细胞系没有任何损害，即使浓度高达400微克/毫升。响应面法用于预测TM和BP的理想协同浓度(EC50)。发现分别为48.5和241.7µg/mL，分别。值得注意的是,与单独暴露于TM相比，选定的条件导致更高的细胞毒性，表明了有效的协同作用。在这种联合治疗下，A549细胞的姜黄素降解速率显着降低至49.72±5.00nmol/h/µg，而NCI-H292细胞则降低至47.53±4.78nmol/h/µg，分别,与单独的姜黄素相比。一起来看,这项研究证实了TM和BP对肺癌细胞系的有效协同作用。需要进一步研究以确定其特定的协同机制。我们的发现为TM和BP的协同作用提供了重要的基础数据。
