PDF(Pubmed)
Abstract：
A novel scaffold design has been created to enhance tissue engineering and regenerative medicine by optimizing the controlled, prolonged release of Hepatocyte Growth Factor (HGF), a powerful chemoattractant for endogenous mesenchymal stem cells. We present a new stacked scaffold that is made up of three different fibrin gel layers, each of which has HGF integrated into the matrix. The design attempts to preserve HGF\'s regenerative properties for long periods of time, which is necessary for complex tissue regeneration. These multi-layered fibrin gels have been mechanically evaluated using rheometry, and their degradation behavior has been studied using D-Dimer ELISA. Understanding the kinetics of HGF release from this novel scaffold configuration is essential for understanding HGF\'s long-term sustained bioactivity. A range of cell-based tests were carried out to verify the functionality of HGF following extended incorporation. These tests included 2-photon microscopy using phalloidin staining to examine cellular morphology, SEM analysis for scaffold-cell interactions, and scratch and scatter assays to assess migration and motility. The analyses show that the novel stacking scaffold promotes vital cellular processes for tissue regeneration in addition to supporting HGF\'s bioactivity. This scaffold design was developed for in situ tissue engineering. Using the body as a bioreactor, the scaffold should recruit mesenchymal stem cells from their niche, thus combining the regenerative abilities of HGF and MSCs to promote tissue remodeling and wound repair.
摘要：
一种新颖的支架设计已经创建，以加强组织工程和再生医学通过优化控制，肝细胞生长因子（HGF）的延长释放，内源性间充质干细胞的强大化学引诱物。我们提出了一种新的堆叠支架，它由三个不同的纤维蛋白凝胶层组成，每个都具有整合到基质中的HGF。该设计试图长时间保持HGF的再生特性，这是复杂组织再生所必需的。这些多层纤维蛋白凝胶已使用流变仪进行了机械评估，并使用D-二聚体ELISA研究了它们的降解行为。了解HGF从这种新型支架结构中释放的动力学对于了解HGF的长期持续生物活性至关重要。进行一系列基于细胞的测试以验证延长掺入后HGF的功能性。这些测试包括2光子显微镜使用鬼笔环肽染色检查细胞形态，支架-细胞相互作用的SEM分析，以及划痕和散射分析，以评估迁移和运动性。分析表明，除了支持HGF的生物活性外，新型堆叠支架还可促进组织再生的重要细胞过程。这种支架设计是为原位组织工程开发的。用身体作为生物反应器，支架应该从它们的小生境中招募间充质干细胞，从而结合HGF和MSCs的再生能力，促进组织重塑和伤口修复。
