PDF(Pubmed)
Abstract：
Recently, we successfully utilized noninvasive magnetic resonance and bioluminescence imaging to track MIN6 cells subcutaneously transplanted in immunocompromised nude mice for up to 64 days. In this study, we further used bioluminescence imaging to investigate the immune rejection of MIN6 cells in immunocompetent C3H mice. A total of 5 × 106 luciferase-transfected MIN6 cells were implanted into the subcutaneous space of each nude or C3H mouse. After transplantation, hypoglycemia and persistent bioluminescence signals were observed in eight of eight (100%) nude mice and five of nine (56%) C3H mice (p < 0.05). We then presensitized a group of C3H mice with C57BL/6 spleen cells just prior to transplantation (n = 14). Interestingly, none of them had hypoglycemia or persistent bioluminescence signals (p < 0.01 vs. C3H mice without presensitization). Histological examination of the grafts revealed a lack or minimal presence of insulin-positive cells in recipients without hypoglycemia and persistent bioluminescence signals. In contrast, recipients with hypoglycemia and persistent bioluminescence signals showed a significant presence of insulin-positive cells in their grafts. Our results indicate that rejection of MIN6 cells occurred in C3H mice and could be enhanced by presensitization with C57BL/6 spleen cells and that bioluminescence imaging is a useful noninvasive tool for detecting rejection of subcutaneously transplanted MIN6 cells.
摘要：
最近,我们成功地利用非侵入性磁共振和生物发光成像技术,追踪在免疫受损裸鼠皮下移植的MIN6细胞长达64天.在这项研究中,我们进一步使用生物发光成像来研究免疫活性C3H小鼠中MIN6细胞的免疫排斥反应。将总共5×106个荧光素酶转染的MIN6细胞植入每只裸小鼠或C3H小鼠的皮下空间。移植后,在8只（100％）裸鼠中的8只和9只（56％）C3H小鼠中的5只（p<0.05）中观察到低血糖和持续的生物发光信号。然后我们在移植前用C57BL/6脾细胞对一组C3H小鼠进行预致敏(n=14)。有趣的是,他们都没有低血糖或持续的生物发光信号(p<0.01vs.没有预敏的C3H小鼠）。移植物的组织学检查显示，在没有低血糖和持续生物发光信号的接受者中，胰岛素阳性细胞的缺乏或极少存在。相比之下,有低血糖和持续性生物发光信号的受者在其移植物中显著存在胰岛素阳性细胞.我们的结果表明，在C3H小鼠中发生了MIN6细胞的排斥反应，并且可以通过C57BL/6脾细胞的预敏作用来增强，并且生物发光成像是检测皮下移植的MIN6细胞排斥反应的有用非侵入性工具。
