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Abstract：
Circulating tumor cells (CTCs) are a type of cancer cell that spreads from the main tumor to the bloodstream, and they are often the most important among the various entities that can be isolated from the blood. For the diagnosis of cancer, conventional biopsies are often invasive and unreliable, whereas a liquid biopsy, which isolates the affected item from blood or lymph fluid, is a less invasive and effective diagnostic technique. Microfluidic technologies offer a suitable channel for conducting liquid biopsies, and this technology is utilized to extract CTCs in a microfluidic chip by physical and bio-affinity-based techniques. This effort uses functionalized magnetic nanoparticles (MNPs) in a unique microfluidic chip to collect CTCs using a hybrid (physical and bio-affinity-based/guided magnetic) capturing approach with a high capture rate. Accordingly, folic acid-functionalized Fe3O4 nanoparticles have been used to capture MCF-7 (breast cancer) CTCs with capture efficiencies reaching up to 95% at a 10 µL/min flow rate. Moreover, studies have been conducted to support this claim, including simulation and biomimetic investigations.
摘要：
循环肿瘤细胞（CTC）是一种从主要肿瘤扩散到血液的癌细胞，它们通常是可以从血液中分离出来的各种实体中最重要的。对于癌症的诊断，传统的活检通常是侵入性的和不可靠的，而液体活检，将受影响的物品与血液或淋巴液隔离，是一种侵入性较小且有效的诊断技术。微流体技术为进行液体活检提供了合适的通道，该技术用于通过基于物理和生物亲和力的技术在微流控芯片中提取CTC。这项工作在独特的微流体芯片中使用功能化的磁性纳米颗粒（MNPs），以使用具有高捕获率的混合（基于物理和生物亲和力的/引导磁性）捕获方法来收集CTC。因此，叶酸功能化的Fe3O4纳米粒子已用于捕获MCF-7（乳腺癌）CTC，在10µL/min的流速下捕获效率高达95％。此外，已经进行了研究来支持这一说法，包括模拟和仿生研究。
