PDF(Pubmed)
Abstract：
Oral cancer represents a significant global public health challenge, contributing substantially to the incidence and mortality of cancer. Despite established risk factors such as tobacco use and alcohol consumption, early detection remains crucial for effective treatment. This study introduces a novel approach using a transistor-based biosensor system for detecting the P90 (CIP2A) protein. We tested the presence of CIP2A in human leukoplakia samples, which can undergo malignant conversion into aggressive oral squamous cell carcinoma. The method used commercially available glucose test strips functionalized with P90 antibodies, providing high sensitivity and a low limit of detection which was five orders lower than that of commercial ELISA kits. A specially designed printed circuit board (PCB) facilitated accurate measurements, and the device\'s performance was optimized through characteristic tests. Human sample testing validated the biosensor\'s effectiveness in distinguishing samples after cell lysis. This study contributes to advancing accurate and cost-effective diagnostic approaches for oral pre-cancer and cancer tissues.
摘要：
口腔癌是一个重大的全球公共卫生挑战。大大有助于癌症的发病率和死亡率。尽管已经确定了吸烟和饮酒等风险因素，早期发现仍然是有效治疗的关键。这项研究介绍了一种使用基于晶体管的生物传感器系统检测P90（CIP2A）蛋白的新方法。我们测试了人类白斑样本中CIP2A的存在，可以恶性转化为侵袭性口腔鳞状细胞癌。该方法使用市售的用P90抗体功能化的葡萄糖测试条，提供高灵敏度和低检测限，比商业ELISA试剂盒低5个数量级。专门设计的印刷电路板(PCB)有助于精确测量，并通过特性测试优化了器件的性能。人体样品测试验证了生物传感器在细胞裂解后区分样品的有效性。这项研究有助于推进口腔癌前病变和癌组织的准确和具有成本效益的诊断方法。
