PDF(Pubmed)
Abstract：
Surface-enhanced Raman spectroscopy (SERS) tagging using silica(SiO2)@Ag nanoparticles (NPs) is easy to handle and is being studied in various fields, including SERS imaging and immunoassays. This is primarily due to its structural advantages, characterized by high SERS activity. However, the Ag NPs introduced onto the SiO2 surface may undergo structural transformation owing to the Ostwald ripening phenomenon under various conditions. As a result, the consistency of the SERS signal decreases, reducing their usability as SERS substrates. Until recently, research has been actively conducted to improve the stability of single Ag NPs. However, research on SiO2@Ag NPs used as a SERS-tagging material is still lacking. In this study, we utilized a Raman labeling compound (RLC) to prevent the structural deformation of SiO2@Ag NPs under various conditions and proposed excellent SiO2@Ag@RLC-Pre NPs as a SERS-tagging material. Using various RLCs, we confirmed that 4-mercaptobenzoic acid (4-MBA) is the RLC that maintains the highest stability for 2 months. These results were also observed for the SiO2@Ag NPs, which were unstable under various pH and temperature conditions. We believe that SERS tags using SiO2@Ag NPs and 4-MBA can be utilized in various applications on based SERS because of the high stability and consistency of the resulting SERS signal.
摘要：
使用二氧化硅（SiO2）@Ag纳米颗粒（NPs）的表面增强拉曼光谱（SERS）标记易于处理，并且正在各个领域进行研究，包括SERS成像和免疫测定。这主要是由于其结构优势，具有高SERS活性。然而,在各种条件下，由于奥斯特瓦尔德熟化现象，引入SiO2表面的AgNP可能会发生结构转变。因此,SERS信号的一致性降低，降低了它们作为SERS基板的可用性。直到最近,已积极进行研究以提高单个AgNPs的稳定性。然而,关于SiO2@AgNPs用作SERS标记材料的研究仍然缺乏。在这项研究中,我们利用拉曼标记化合物（RLC）来防止SiO2@AgNPs在各种条件下的结构变形，并提出了出色的SiO2@Ag@RLC-PreNPs作为SERS标记材料。使用各种RLCs，我们证实,4-巯基苯甲酸(4-MBA)是保持2个月最高稳定性的RLC。这些结果也观察到SiO2@AgNPs，在各种pH和温度条件下不稳定。我们相信使用SiO2@AgNP和4-MBA的SERS标签可以用于基于SERS的各种应用中,因为所得SERS信号的高稳定性和一致性。
