Abstract：
Aim: To investigate the effect of surfactant type on curcumin-loaded (CUR) PLGA nanoparticles (NPs) to modulate monocyte functions. Materials & methods: The nanoprecipitation method was used, and PLGA NPs were designed using Pluronic F127 (F127) and/or lecithin (LEC) as surfactants. Results: The Z-average of the NPs was <200 nm, they had a spherical shape, Derjaguin-Muller-Toporov modulus >0.128 MPa, they were stable during storage at 4°C, ζ-potential ∼-40 mV, polydispersity index <0.26 and % EE of CUR >94%. PLGA-LEC/F127 NPs showed favorable physicochemical and nanomechanical properties. These NPs were bound and internalized mainly by monocytes, suppressed monocyte-induced reactive oxygen species production, and decreased the ability of monocytes to modulate T-cell proliferation. Conclusion: These results demonstrate the potential of these NPs for targeted therapy.
This study explores how different surfactants affect curcumin-loaded PLGA nanoparticles, a biodegradable polymer. The nanoparticles were designed using Pluronic F127 and/or lecithin as surfactants. They are less than 200 nm and spherical. They are stable when stored at 4 °C, with a surface charge of about -40 mV, and can encapsulate more than 94% of curcumin.The results of this study are promising, showing that PLGA nanoparticles using a mixture of lecithin and Pluronic F127 as surfactants have favorable properties toward monocyte adhesion. They are primarily taken up by monocytes, a type of white blood cell, and demonstrate a remarkable ability to reduce the production of reactive oxygen species, which can cause cell damage, as well as the ability of monocytes to stimulate the proliferation of T cells. This underscores the potential of these nanoparticles in targeted therapy, particularly in diseases where monocytes play a pivotal role, such as chronic inflammatory conditions.
This study explores how different surfactants affect curcumin-loaded PLGA nanoparticles, a biodegradable polymer. The nanoparticles were designed using Pluronic F127 and/or lecithin as surfactants. They are less than 200 nm and spherical. They are stable when stored at 4 °C, with a surface charge of about -40 mV, and can encapsulate more than 94% of curcumin.The results of this study are promising, showing that PLGA nanoparticles using a mixture of lecithin and Pluronic F127 as surfactants have favorable properties toward monocyte adhesion. They are primarily taken up by monocytes, a type of white blood cell, and demonstrate a remarkable ability to reduce the production of reactive oxygen species, which can cause cell damage, as well as the ability of monocytes to stimulate the proliferation of T cells. This underscores the potential of these nanoparticles in targeted therapy, particularly in diseases where monocytes play a pivotal role, such as chronic inflammatory conditions.
摘要：
目的：研究表面活性剂类型对姜黄素（CUR）PLGA纳米颗粒（NPs）调节单核细胞功能的影响。材料和方法：使用纳米沉淀法，使用PluronicF127(F127)和/或卵磷脂(LEC)作为表面活性剂设计PLGANP。结果:NPs的Z-平均值<200nm,它们是球形的，Derjaguin-Muller-Toporov模量>0.128MPa,它们在4℃储存期间是稳定的，ζ-电位~-40mV，多分散指数<0.26和CUR的%EE>94%。PLGA-LEC/F127NP表现出良好的物理化学和纳米力学性能。这些NP主要被单核细胞结合和内化，抑制单核细胞诱导的活性氧产生，并降低单核细胞调节T细胞增殖的能力。结论:这些结果证明了这些NP用于靶向治疗的潜力。
这项研究探讨了不同的表面活性剂如何影响姜黄素负载的PLGA纳米颗粒，一种可生物降解的聚合物.使用PluronicF127和/或卵磷脂作为表面活性剂设计纳米颗粒。它们小于200nm并且是球形的。它们在4°C下储存时是稳定的，表面电荷约为-40mV,并且可以包封超过94%的姜黄素。这项研究的结果是有希望的，显示使用卵磷脂和PluronicF127的混合物作为表面活性剂的PLGA纳米颗粒对单核细胞粘附具有有利的性质。它们主要被单核细胞吸收，一种白细胞,并表现出显著的减少活性氧产生的能力，会导致细胞损伤，以及单核细胞刺激T细胞增殖的能力。这强调了这些纳米粒子在靶向治疗中的潜力，特别是在单核细胞发挥关键作用的疾病中，如慢性炎症。
这项研究探讨了不同的表面活性剂如何影响姜黄素负载的PLGA纳米颗粒，一种可生物降解的聚合物.使用PluronicF127和/或卵磷脂作为表面活性剂设计纳米颗粒。它们小于200nm并且是球形的。它们在4°C下储存时是稳定的，表面电荷约为-40mV,并且可以包封超过94%的姜黄素。这项研究的结果是有希望的，显示使用卵磷脂和PluronicF127的混合物作为表面活性剂的PLGA纳米颗粒对单核细胞粘附具有有利的性质。它们主要被单核细胞吸收，一种白细胞,并表现出显著的减少活性氧产生的能力，会导致细胞损伤，以及单核细胞刺激T细胞增殖的能力。这强调了这些纳米粒子在靶向治疗中的潜力，特别是在单核细胞发挥关键作用的疾病中，如慢性炎症。
