Abstract：
UNASSIGNED: Preconditioning by intermittent fasting is linked to improved cognition and motor function, and enhanced recovery after stroke. Although the duration of fasting was shown to elicit different levels of neuroprotection after ischemic stroke, the impact of time of fasting with respect to the circadian cycles remains unexplored.
UNASSIGNED: Cohorts of mice were subjected to a daily 16-hour fast, either during the dark phase (active-phase intermittent fasting) or the light phase (inactive-phase intermittent fasting) or were fed ad libitum. Following a 6-week dietary regimen, mice were subjected to transient focal cerebral ischemia and underwent behavioral functional assessment. Brain samples were collected for RNA sequencing and histopathologic analyses.
UNASSIGNED: Active-phase intermittent fasting cohort exhibited better poststroke motor and cognitive recovery as well as reduced infarction, in contrast to inactive-phase intermittent fasting cohort, when compared with ad libitum cohort. In addition, protection of dendritic spine density/morphology and increased expression of postsynaptic density protein-95 were observed in the active-phase intermittent fasting.
UNASSIGNED: These findings indicate that the time of daily fasting is an important factor in inducing ischemic tolerance by intermittent fasting.
UNASSIGNED: Cohorts of mice were subjected to a daily 16-hour fast, either during the dark phase (active-phase intermittent fasting) or the light phase (inactive-phase intermittent fasting) or were fed ad libitum. Following a 6-week dietary regimen, mice were subjected to transient focal cerebral ischemia and underwent behavioral functional assessment. Brain samples were collected for RNA sequencing and histopathologic analyses.
UNASSIGNED: Active-phase intermittent fasting cohort exhibited better poststroke motor and cognitive recovery as well as reduced infarction, in contrast to inactive-phase intermittent fasting cohort, when compared with ad libitum cohort. In addition, protection of dendritic spine density/morphology and increased expression of postsynaptic density protein-95 were observed in the active-phase intermittent fasting.
UNASSIGNED: These findings indicate that the time of daily fasting is an important factor in inducing ischemic tolerance by intermittent fasting.
摘要：
■间歇性禁食的预处理与改善认知和运动功能有关，增强中风后的恢复。尽管显示禁食的持续时间在缺血性卒中后引起不同程度的神经保护,禁食时间对昼夜节律周期的影响仍未被探索。
■成群的小鼠每天禁食16小时，在黑暗阶段（活动期间歇性禁食）或光阶段（非活动期间歇性禁食）或随意喂食。经过6周的饮食方案，对小鼠进行短暂性局灶性脑缺血并进行行为功能评估。收集脑样品用于RNA测序和组织病理学分析。
■活跃期间歇性禁食队列显示出更好的卒中后运动和认知恢复以及减少的梗死，与非活动期间歇性禁食队列相反，与随意队列相比。此外,在活动期间歇性禁食中观察到树突棘密度/形态的保护和突触后密度蛋白95的表达增加。
■这些发现表明，每日禁食的时间是通过间歇性禁食诱导缺血耐受的重要因素。
■成群的小鼠每天禁食16小时，在黑暗阶段（活动期间歇性禁食）或光阶段（非活动期间歇性禁食）或随意喂食。经过6周的饮食方案，对小鼠进行短暂性局灶性脑缺血并进行行为功能评估。收集脑样品用于RNA测序和组织病理学分析。
■活跃期间歇性禁食队列显示出更好的卒中后运动和认知恢复以及减少的梗死，与非活动期间歇性禁食队列相反，与随意队列相比。此外,在活动期间歇性禁食中观察到树突棘密度/形态的保护和突触后密度蛋白95的表达增加。
■这些发现表明，每日禁食的时间是通过间歇性禁食诱导缺血耐受的重要因素。
