PDF(Pubmed)
Abstract：
During infection, positive-stranded RNA causes a rearrangement of the host cell membrane, resulting in specialized membrane structure formation aiding viral genome replication. Double-membrane vesicles (DMVs), typical structures produced by virus-induced membrane rearrangements, are platforms for viral replication. Nidoviruses, one of the most complex positive-strand RNA viruses, have the ability to infect not only mammals and a few birds but also invertebrates. Nidoviruses possess a distinctive replication mechanism, wherein their nonstructural proteins (nsps) play a crucial role in DMV biogenesis. With the participation of host factors related to autophagy and lipid synthesis pathways, several viral nsps hijack the membrane rearrangement process of host endoplasmic reticulum (ER), Golgi apparatus, and other organelles to induce DMV formation. An understanding of the mechanisms of DMV formation and its structure and function in the infectious cycle of nidovirus may be essential for the development of new and effective antiviral strategies in the future.
摘要：
在感染期间,正链RNA引起宿主细胞膜的重排，导致专门的膜结构形成，帮助病毒基因组复制。双膜囊泡（DMV），病毒诱导的膜重排产生的典型结构，是病毒复制的平台。Nidovirus,最复杂的正链RNA病毒之一，不仅有能力感染哺乳动物和少数鸟类，而且有能力感染无脊椎动物。Nidovirus具有独特的复制机制，其中它们的非结构蛋白（nsps）在DMV生物发生中起着至关重要的作用。在自噬和脂质合成通路相关宿主因子的参与下,几种病毒NSP劫持了宿主内质网（ER）的膜重排过程，高尔基体,和其他细胞器诱导DMV形成。了解DMV的形成机制及其在Nidovirus感染周期中的结构和功能对于将来开发新的有效抗病毒策略至关重要。
