PDF(Pubmed)
Abstract：
UNASSIGNED: The study assessed the correlation and concordance of 25-hydroxyvitamin D [25(OH)D] levels in capillary and venous plasma collected simultaneously after vitamin D3 supplementation in 42 healthy adults. They were randomly divided into three groups by random number table method. Group A took 1,000 IU vitamin D3 daily, group B took 10,000 IU vitamin D3 every 10 days, and group C took 30,000 IU vitamin D3 every 30 days until the end of the 12th month. Venous blood serum 25(OH)D level was detected by chemiluminescence immunoassay (CLIA) and mass spectrometry (LC-MS) at day 1, day 14, day 28, month 6, and month 12 respectively, the capillary blood serum 25(OH)D level was detected by chemiluminescence immunoassay (CLIA) at the same time. Pearson correlation analysis and linear regression analysis were employed to investigate the relationship and transformation equation between the findings of the two samples and the results obtained from different detection methods within the same sample. The Bland-Altman method, Kappa analysis, and receiver operating characteristic (ROC) curve were utilized for assessing consistency, sensitivity, and specificity.
UNASSIGNED: The three groups all reached a stable peak at 6 months, and the average levels of the three groups were 49.21, 42.50 and 43.025 nmol/L, respectively. The average levels of group A were higher than those of group B and group C (P < 0.001). The mean values of serum 25(OH)D measured by LC-MS and CLIA in 42 healthy adults were 45.32 nmol/L and 49.88 nmol/L, respectively, and the mean values of 25(OH)D measured by LC-MS in capillary blood were 52.03 nmol/L, and the difference was statistically significant (P < 0.001). Pearson correlation analysis showed that the linear fitting formula of scatter data was as follows: venous 25(OH)D concentration (nmol/L) = 1.105 * capillary 25(OH)D concentration -7.532 nmol/L, R2 = 0.625. Good agreement was observed between venous and corrected capillary 25(OH)D levels in clinical diagnosis (Kappa value 0.75). The adjusted serum 25(OH)D in capillary blood had a high clinical predictive value.
UNASSIGNED: The agreement between the two methods is good when the measured 25(OH)D level is higher. Standardized capillary blood chemiluminescence method can be used for 25(OH)D detection.
UNASSIGNED: The three groups all reached a stable peak at 6 months, and the average levels of the three groups were 49.21, 42.50 and 43.025 nmol/L, respectively. The average levels of group A were higher than those of group B and group C (P < 0.001). The mean values of serum 25(OH)D measured by LC-MS and CLIA in 42 healthy adults were 45.32 nmol/L and 49.88 nmol/L, respectively, and the mean values of 25(OH)D measured by LC-MS in capillary blood were 52.03 nmol/L, and the difference was statistically significant (P < 0.001). Pearson correlation analysis showed that the linear fitting formula of scatter data was as follows: venous 25(OH)D concentration (nmol/L) = 1.105 * capillary 25(OH)D concentration -7.532 nmol/L, R2 = 0.625. Good agreement was observed between venous and corrected capillary 25(OH)D levels in clinical diagnosis (Kappa value 0.75). The adjusted serum 25(OH)D in capillary blood had a high clinical predictive value.
UNASSIGNED: The agreement between the two methods is good when the measured 25(OH)D level is higher. Standardized capillary blood chemiluminescence method can be used for 25(OH)D detection.
摘要：
■该研究评估了42名健康成年人补充维生素D3后同时收集的毛细血管和静脉血浆中25-羟基维生素D[25（OH）D]水平的相关性和一致性。采用随机数字表法将其随机分为3组。A组每天服用1000IU维生素D3，B组每10天服用10,000IU维生素D3，C组每30天服用30,000IU维生素D3，直到第12个月结束。分别于第1天、第14天、第28天、第6个月和第12个月采用化学发光免疫分析法(CLIA)和质谱(LC-MS)检测血清25(OH)D水平,同时用化学发光免疫分析法(CLIA)检测毛细血管血清25(OH)D水平。采用Pearson相关分析和线性回归分析研究了两种样品的发现与同一样品中不同检测方法获得的结果之间的关系和转换方程。Bland-Altman方法,Kappa分析,和受试者工作特征(ROC)曲线用于评估一致性，灵敏度,和特异性。
■三组均在6个月时达到稳定高峰，三组的平均水平分别为49.21、42.50和43.025nmol/L，分别。A组的平均水平高于B组和C组（P<0.001）。LC-MS和CLIA检测42例健康成人血清25(OH)D平均值分别为45.32nmol/L和49.88nmol/L,分别,LC-MS测定毛细血管血中25(OH)D的平均值为52.03nmol/L,差异有统计学意义(P<0.001)。Pearson相关分析表明,散点图数据的线性拟合公式为:静脉25(OH)D浓度(nmol/L)=1.105*毛细血管25(OH)D浓度-7.532nmol/L,R2=0.625。在临床诊断中，静脉和校正的毛细血管25（OH）D水平之间观察到良好的一致性（Kappa值0.75）。毛细血管血中调整的血清25(OH)D具有较高的临床预测价值。
■当测得的25（OH）D水平较高时，两种方法之间的一致性很好。标准化毛细管血液化学发光法可用于25(OH)D的检测。
■三组均在6个月时达到稳定高峰，三组的平均水平分别为49.21、42.50和43.025nmol/L，分别。A组的平均水平高于B组和C组（P<0.001）。LC-MS和CLIA检测42例健康成人血清25(OH)D平均值分别为45.32nmol/L和49.88nmol/L,分别,LC-MS测定毛细血管血中25(OH)D的平均值为52.03nmol/L,差异有统计学意义(P<0.001)。Pearson相关分析表明,散点图数据的线性拟合公式为:静脉25(OH)D浓度(nmol/L)=1.105*毛细血管25(OH)D浓度-7.532nmol/L,R2=0.625。在临床诊断中，静脉和校正的毛细血管25（OH）D水平之间观察到良好的一致性（Kappa值0.75）。毛细血管血中调整的血清25(OH)D具有较高的临床预测价值。
■当测得的25（OH）D水平较高时，两种方法之间的一致性很好。标准化毛细管血液化学发光法可用于25(OH)D的检测。
