Abstract：
BACKGROUND: Tongue cancer is the most prevalent type of oral cancer. Recently, natural compounds have been considered important resources for several anticancer drugs. Thymoquinone (TQ) exhibits a potent anti-cancer effect. 5-Fluorouracil (5-FU) is a chemotherapeutic drug that has been utilized in the treatment of cancer. Recently, combination therapy has gained popularity as a treatment option for patients with cancer.
OBJECTIVE: The present study was carried out to assess the cytotoxic effect of 5-Fluorouracil (5-FU), Thymoquinone (TQ), and their combination on tongue squamous cell carcinoma cell line (HNO-97).
METHODS: Tongue carcinoma cell line (HNO-97) was maintained in cultured flasks and the cells were divided into four groups; group Ι: control untreated group, group ΙΙ: HNO-97-treated cells with different concentrations of 5-FU from 0.5 µM/ml to 3µM/ml, group ΙIΙ: HNO-97-treated cells with different concentrations of TQ from 7.25µM/ml to 23.05µM/ml, and group ΙV: HNO-97-treated cells with both 5-FU and TQ in serial concentrations till (IC50) in a dose of 27.44 µM/ml. Determination of the cytotoxic effect of the tested agents on the HNO-97 cell line was done using methyl thiazole tetrazolium assay, nuclear morphometric analysis, microscopic examination, and annexin-v/ propidium iodide staining assay.
RESULTS: The findings revealed that the cytotoxic effect of 5-FU, TQ, and their combination on tongue squamous cell carcinoma cell line (HNO-97) was dose-dependent. The microscopic examination revealed that 5-FU, TQ alone, or their combination induced apoptotic cell death. P-value < 0.05 was statistically significant.
CONCLUSIONS: The combination of 5-FU and TQ produced a marked cytotoxic effect on HNO-97 cells.
OBJECTIVE: The present study was carried out to assess the cytotoxic effect of 5-Fluorouracil (5-FU), Thymoquinone (TQ), and their combination on tongue squamous cell carcinoma cell line (HNO-97).
METHODS: Tongue carcinoma cell line (HNO-97) was maintained in cultured flasks and the cells were divided into four groups; group Ι: control untreated group, group ΙΙ: HNO-97-treated cells with different concentrations of 5-FU from 0.5 µM/ml to 3µM/ml, group ΙIΙ: HNO-97-treated cells with different concentrations of TQ from 7.25µM/ml to 23.05µM/ml, and group ΙV: HNO-97-treated cells with both 5-FU and TQ in serial concentrations till (IC50) in a dose of 27.44 µM/ml. Determination of the cytotoxic effect of the tested agents on the HNO-97 cell line was done using methyl thiazole tetrazolium assay, nuclear morphometric analysis, microscopic examination, and annexin-v/ propidium iodide staining assay.
RESULTS: The findings revealed that the cytotoxic effect of 5-FU, TQ, and their combination on tongue squamous cell carcinoma cell line (HNO-97) was dose-dependent. The microscopic examination revealed that 5-FU, TQ alone, or their combination induced apoptotic cell death. P-value < 0.05 was statistically significant.
CONCLUSIONS: The combination of 5-FU and TQ produced a marked cytotoxic effect on HNO-97 cells.
摘要：
背景：舌癌是口腔癌中最常见的类型。最近,天然化合物被认为是几种抗癌药物的重要资源。胸醌(TQ)表现出有效的抗癌作用。5-氟尿嘧啶（5-FU）是一种化疗药物，已用于治疗癌症。最近,联合治疗已成为癌症患者的治疗选择。
目的：本研究旨在评估5-氟尿嘧啶（5-FU）的细胞毒性作用，百里酚(TQ),以及它们在舌鳞状细胞癌细胞系（HNO-97）上的组合。
方法：将舌癌细胞株（HNO-97）置于培养瓶中，分为4组；组1：对照组，II组：HNO-97处理的细胞，具有0.5µM/ml至3µM/ml的不同浓度的5-FU，III组：HNO-97处理的细胞，具有从7.25µM/ml至23.05µM/ml的不同浓度的TQ，和IV组:以27.44μM/ml的剂量用连续浓度的5-FU和TQ(IC50)处理的细胞。使用甲基噻唑四唑盐测定法确定受试药物对HNO-97细胞系的细胞毒性作用，核形态分析,显微镜检查,和膜联蛋白-v/碘化丙啶染色测定。
结果：研究结果表明，5-FU的细胞毒性作用，TQ,它们在舌鳞状细胞癌细胞系（HNO-97）上的组合呈剂量依赖性。显微镜检查显示5-FU，TQ独自一人，或它们的组合诱导凋亡细胞死亡。P值<0.05有统计学意义。
结论:5-FU和TQ的组合对HNO-97细胞产生显著的细胞毒性作用。
目的：本研究旨在评估5-氟尿嘧啶（5-FU）的细胞毒性作用，百里酚(TQ),以及它们在舌鳞状细胞癌细胞系（HNO-97）上的组合。
方法：将舌癌细胞株（HNO-97）置于培养瓶中，分为4组；组1：对照组，II组：HNO-97处理的细胞，具有0.5µM/ml至3µM/ml的不同浓度的5-FU，III组：HNO-97处理的细胞，具有从7.25µM/ml至23.05µM/ml的不同浓度的TQ，和IV组:以27.44μM/ml的剂量用连续浓度的5-FU和TQ(IC50)处理的细胞。使用甲基噻唑四唑盐测定法确定受试药物对HNO-97细胞系的细胞毒性作用，核形态分析,显微镜检查,和膜联蛋白-v/碘化丙啶染色测定。
结果：研究结果表明，5-FU的细胞毒性作用，TQ,它们在舌鳞状细胞癌细胞系（HNO-97）上的组合呈剂量依赖性。显微镜检查显示5-FU，TQ独自一人，或它们的组合诱导凋亡细胞死亡。P值<0.05有统计学意义。
结论:5-FU和TQ的组合对HNO-97细胞产生显著的细胞毒性作用。
