Abstract：
OBJECTIVE: Our study aimed to compare aquaporin profiles in advanced and early passage bone marrow-derived mesenchymal stem cells (BM-MSCs) and assess the impact of aquaporin changes after adipogenic differentiation. Aquaporins are crucial for stem cell survival and differentiation during their life cycle. We focused on the role of aquaporins in the cell structures of advanced and early passage stem cells.
METHODS: In our study, BM-MSCs were used for our objectives. Characterization of the cells was evaluated via flow cytometry using stem cell surface markers. The characterized BM-MSCs were divided into control and differentiation groups at passages 3 (P3) and 8 (P8). AQP1, AQP3, AQP7, AQP9, and AQP10 expression levels on days 0, 1, 3, 7, 14, and 21 were evaluated using Real Time-PCR, ELISA, and immunofluorescence studies.
RESULTS: The cells were characterized by flow cytometry and confirmed to exhibit BM-MSC characteristics. At P3 and P8, differentiation was initiated, and AQP protein expression was observed to initially increase and then decrease on subsequent days. The increase in AQP protein expression at P3 occurred earlier than that at P8. Gene expression analysis demonstrated a statistically significant increase in AQP gene expression on days when AQP protein expression decreased. Moreover, statistical differences were observed between late and early passage AQP profiles.
CONCLUSIONS: Our study examined the composition of AQPs in BM-MSCs in association with cell passage, and found that AQPs play a role in the differentiation process. The connection between the AQP profile and aging might be related to differentiation capacity, which could have implications for slowing down cellular aging and developing new therapeutic approaches.
METHODS: In our study, BM-MSCs were used for our objectives. Characterization of the cells was evaluated via flow cytometry using stem cell surface markers. The characterized BM-MSCs were divided into control and differentiation groups at passages 3 (P3) and 8 (P8). AQP1, AQP3, AQP7, AQP9, and AQP10 expression levels on days 0, 1, 3, 7, 14, and 21 were evaluated using Real Time-PCR, ELISA, and immunofluorescence studies.
RESULTS: The cells were characterized by flow cytometry and confirmed to exhibit BM-MSC characteristics. At P3 and P8, differentiation was initiated, and AQP protein expression was observed to initially increase and then decrease on subsequent days. The increase in AQP protein expression at P3 occurred earlier than that at P8. Gene expression analysis demonstrated a statistically significant increase in AQP gene expression on days when AQP protein expression decreased. Moreover, statistical differences were observed between late and early passage AQP profiles.
CONCLUSIONS: Our study examined the composition of AQPs in BM-MSCs in association with cell passage, and found that AQPs play a role in the differentiation process. The connection between the AQP profile and aging might be related to differentiation capacity, which could have implications for slowing down cellular aging and developing new therapeutic approaches.
摘要：
目的：我们的研究旨在比较晚期和早期传代骨髓间充质干细胞（BM-MSCs）的水通道蛋白分布，并评估成脂分化后水通道蛋白变化的影响。水通道蛋白在其生命周期中对于干细胞存活和分化至关重要。我们专注于水通道蛋白在高级和早期传代干细胞的细胞结构中的作用。
方法：在我们的研究中，BM-MSC用于我们的目标。使用干细胞表面标志物通过流式细胞术评估细胞的表征。将表征的BM-MSC在第3代(P3)和第8代(P8)分为对照组和分化组。使用RealTime-PCR评估第0、1、3、7、14和21天的AQP1，AQP3，AQP7，AQP9和AQP10表达水平，ELISA,和免疫荧光研究。
结果：通过流式细胞术对细胞进行表征，并证实显示BM-MSC特征。在P3和P8开始分化，观察到AQP蛋白表达最初增加，然后在随后的几天减少。P3时AQP蛋白表达的增加比P8时更早。基因表达分析表明，当AQP蛋白表达降低时，AQP基因表达在统计学上显着增加。此外，在晚期和早期传代AQP谱之间观察到统计学差异.
结论：我们的研究检查了与细胞传代相关的BM-MSCs中AQPs的组成，并发现AQPs在分化过程中发挥作用。AQP概况与衰老之间的联系可能与分化能力有关，这可能对减缓细胞衰老和开发新的治疗方法有影响。
方法：在我们的研究中，BM-MSC用于我们的目标。使用干细胞表面标志物通过流式细胞术评估细胞的表征。将表征的BM-MSC在第3代(P3)和第8代(P8)分为对照组和分化组。使用RealTime-PCR评估第0、1、3、7、14和21天的AQP1，AQP3，AQP7，AQP9和AQP10表达水平，ELISA,和免疫荧光研究。
结果：通过流式细胞术对细胞进行表征，并证实显示BM-MSC特征。在P3和P8开始分化，观察到AQP蛋白表达最初增加，然后在随后的几天减少。P3时AQP蛋白表达的增加比P8时更早。基因表达分析表明，当AQP蛋白表达降低时，AQP基因表达在统计学上显着增加。此外，在晚期和早期传代AQP谱之间观察到统计学差异.
结论：我们的研究检查了与细胞传代相关的BM-MSCs中AQPs的组成，并发现AQPs在分化过程中发挥作用。AQP概况与衰老之间的联系可能与分化能力有关，这可能对减缓细胞衰老和开发新的治疗方法有影响。
