Abstract：
Different types of pathogenic viruses that have common transmission path can be co-infected, inducing distinct disease procession in comparison to that infection of one. Also, in the post COVID-19 time, more types of respiratory infectious virus are becoming prevalent and are concurrent. Those bring an urgent need for detection of co-existing viruses. Here, we propose a visualized lateral flow assay for logic determination of co-existing viral RNA fragments. In the presence of specific viral RNA inputs, DNAzyme is de-blocked according to defined logic, and catalyzes the hydrolysis of hairpin-structural substrate. One of cleaved substrates contains DNAzyme domain to realize dual signal amplification, which obtains copious of the other cleaved substrates. The cleaved substrates act as linking strands for bridging DNA-modified gold nanoparticles onto lateral flow strip to induce coloration on test line. \"AND\", \"OR\" and \"INHIBIT\" controlled lateral flow assays are respectively demonstrated for co-existing viral RNA detection, and the visual results can be obtained by the same kind of prepared strip, without need of re-fabricating strips according to logic systems. The work provides a flexible, convenient, visual and logic-processing strategy for simultaneous analysis of co-existing viruses.
摘要：
具有共同传播途径的不同类型的致病病毒可以共同感染,与一个人的感染相比，诱导不同的疾病进程。此外，在后COVID-19时间，更多类型的呼吸道感染性病毒正在变得流行和并发。这些迫切需要检测共存的病毒。这里,我们提出了一种可视化的侧流测定法，用于逻辑确定共存的病毒RNA片段。在存在特定病毒RNA输入的情况下，DNAzyme根据定义的逻辑被解除阻塞，并催化发夹结构底物的水解。其中一个切割的底物含有DNAzyme结构域以实现双信号放大，它获得了大量的其他裂解的底物。切割的底物充当用于将DNA修饰的金纳米颗粒桥接到横向流动条上的连接链,以诱导测试线上的着色。\"AND\",\"OR\"和\"INHIBIT\"控制侧流测定分别证明共存的病毒RNA检测，并且可以通过相同类型的制备条获得视觉结果，无需根据逻辑系统重新制造条带。这项工作提供了一个灵活的，方便,同时分析共存病毒的视觉和逻辑处理策略。
