Abstract:
A method is presented for chemiluminescence resonance energy transfer (CRET) using APTES-Fe3O4 as a highly efficient energy acceptor with strong magnetic effectiveness over extended distances, while an Au@BSA-luminol composite acts as the donor. In order to boost the chemiluminescence reactions, CuO nanoparticles were successfully employed. The distance between the donor and acceptor is a crucial factor in the occurrence of the CRET phenomenon. A sensitive and high-throughput sandwich chemiluminescence immunosensor has been developed accordingly with a linear range of 1.0 × 10-7 g/L to 6.0 × 10-5 g/L and a limit of detection of 0.8 × 10-7 g/L. The CRET-based sandwich immunosensor has the potential to be implemented to early cancer diagnosis because of its high sensitivity in detecting Nanog, fast analysis (30 min), and simplicity. Furthermore, this approach has the potential to be adapted for the recognition of other antigen-antibody immune complexes by utilizing the corresponding antigens and their selective antibodies.
摘要:
提出了一种使用APTES-Fe3O4作为高效能量受体的化学发光共振能量转移(CRET)的方法,该方法在很长的距离上具有很强的磁效力,而Au@BSA-鲁米诺复合材料充当供体。为了促进化学发光反应,成功地使用了CuO纳米颗粒。供体和受体之间的距离是发生CRET现象的关键因素。相应地开发了灵敏和高通量的夹心化学发光免疫传感器,线性范围为1.0×10-7g/L至6.0×10-5g/L,检出限为0.8×10-7g/L。基于CRET的夹心免疫传感器具有检测Nanog的高灵敏度,因此有可能用于早期癌症诊断。快速分析(30分钟),和简单。此外,这种方法有可能通过利用相应的抗原及其选择性抗体来适应其他抗原-抗体免疫复合物的识别。