PDF(Pubmed)
Abstract：
In recent years, most studies on the gut microbiome have primarily focused on feces samples, leaving the microbial communities in the intestinal mucosa relatively unexplored. To address this gap, our study employed shotgun metagenomics to analyze the microbial compositions in normal rectal mucosa and matched feces from 20 patients with colonic polyps. Our findings revealed a pronounced distinction of the microbial communities between these two sample sets. Compared with feces, the mucosal microbiome contains fewer genera, with Burkholderia being the most discriminating genus between feces and mucosa, highlighting its significant influence on the mucosa. Furthermore, based on the microbial classification and KEGG Orthology (KO) annotation results, we explored the association between rectal mucosal microbiota and factors such as age, gender, BMI, and polyp risk level. Notably, we identified novel biomarkers for these phenotypes, such as Clostridium ramosum and Enterobacter cloacae in age. The mucosal microbiota showed an enrichment of KO pathways related to sugar transport and short chain fatty acid metabolism. Our comprehensive approach not only bridges the knowledge gap regarding the microbial community in the rectal mucosa but also underscores the complexity and specificity of microbial interactions within the human gut, particularly in the Chinese population.
OBJECTIVE: This study presents a system-level map of the differences between feces and rectal mucosal microbial communities in samples with colorectal cancer risk. It reveals the unique microecological characteristics of rectal mucosa and its potential influence on health. Additionally, it provides novel insights into the role of the gut microbiome in the pathogenesis of colorectal cancer and paves the way for the development of new prevention and treatment strategies.
OBJECTIVE: This study presents a system-level map of the differences between feces and rectal mucosal microbial communities in samples with colorectal cancer risk. It reveals the unique microecological characteristics of rectal mucosa and its potential influence on health. Additionally, it provides novel insights into the role of the gut microbiome in the pathogenesis of colorectal cancer and paves the way for the development of new prevention and treatment strategies.
摘要：
近年来,大多数关于肠道微生物组的研究主要集中在粪便样本上，使肠粘膜中的微生物群落相对未被探索。为了解决这个差距，我们的研究使用鸟枪宏基因组学分析了20例结肠息肉患者的正常直肠粘膜和匹配粪便中的微生物组成。我们的发现揭示了这两个样本集之间微生物群落的明显区别。与粪便相比，粘膜微生物组包含较少的属，伯克霍尔德菌是粪便和粘膜之间最有区别的属，强调其对粘膜的显著影响。此外，基于微生物分类和KEGG骨科(KO)成果注解，我们探讨了直肠粘膜微生物群与年龄等因素之间的关系，性别,BMI,和息肉风险水平。值得注意的是,我们确定了这些表型的新生物标志物，例如在年龄上有拉氏梭状芽胞杆菌和阴沟肠杆菌。粘膜微生物群显示与糖转运和短链脂肪酸代谢相关的KO途径的富集。我们的综合方法不仅弥合了有关直肠粘膜微生物群落的知识鸿沟，而且还强调了人体肠道内微生物相互作用的复杂性和特异性。特别是在中国人口中。
目的：本研究提供了具有结直肠癌风险的样本中粪便和直肠粘膜微生物群落之间差异的系统水平图。它揭示了直肠粘膜独特的微生态特征及其对健康的潜在影响。此外,它提供了对肠道微生物组在结直肠癌发病机制中的作用的新见解，并为开发新的预防和治疗策略铺平了道路。
目的：本研究提供了具有结直肠癌风险的样本中粪便和直肠粘膜微生物群落之间差异的系统水平图。它揭示了直肠粘膜独特的微生态特征及其对健康的潜在影响。此外,它提供了对肠道微生物组在结直肠癌发病机制中的作用的新见解，并为开发新的预防和治疗策略铺平了道路。
