PDF(Pubmed)
Abstract：
Extracellular vesicles (EVs) are microparticles released from cells in both physiological and pathological conditions and could be used to monitor the progression of various pathological states, including neoplastic diseases. In various EVs, tumor-derived extracellular vesicles (TEVs) are secreted by different tumor cells and are abundant in many molecular components, such as proteins, nucleic acids, lipids, and carbohydrates. TEVs play a crucial role in forming and advancing various cancer processes. Therefore, TEVs are regarded as promising biomarkers for the early detection of cancer in liquid biopsy. However, the currently developed TEV detection methods still face several key scientific problems that need to be solved, such as low sensitivity, poor specificity, and poor accuracy. To overcome these limitations, DNA walkers have emerged as one of the most popular nanodevices that exhibit better signal amplification capability and enable highly sensitive and specific detection of the analytes. Due to their unique properties of high directionality, flexibility, and efficiency, DNA walkers hold great potential for detecting TEVs. This paper provides an introduction to EVs and DNA walker, additionally, it summarizes recent advances in DNA walker-based detection of TEVs (2018-2024). The review highlights the close relationship between TEVs and DNA walkers, aims to offer valuable insights into TEV detection and to inspire the development of reliable, efficient, simple, and innovative methods for detecting TEVs based on DNA walker in the future.
摘要：
细胞外囊泡（EV）是在生理和病理条件下从细胞释放的微粒，可用于监测各种病理状态的进展。包括肿瘤疾病。在各种电动汽车中，肿瘤来源的细胞外囊泡（TEV）由不同的肿瘤细胞分泌，并且在许多分子成分中丰富，如蛋白质，核酸,脂质，和碳水化合物。TEV在形成和推进各种癌症过程中起着至关重要的作用。因此,TEV被认为是在液体活检中早期检测癌症的有前途的生物标志物。然而,目前发展起来的TEV检测方法还面临着需要解决的几个关键科学问题,如灵敏度低，特异性差，精度差。为了克服这些限制,DNA助行器已成为最流行的纳米器件之一，其表现出更好的信号放大能力，并能够对分析物进行高灵敏度和特异性检测。由于它们具有高方向性的独特特性，灵活性,和效率，DNA行走者具有检测TEV的巨大潜力。本文介绍了电动汽车和DNA步行者，此外，它总结了基于DNAWalker的TEV检测的最新进展(2018-2024)。该评论强调了TEV与DNA步行者之间的密切关系，旨在为TEV检测提供有价值的见解，并激发可靠的发展，高效,简单,以及未来基于DNAWalker检测TEV的创新方法。
