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Abstract：
UNASSIGNED: The current study aimed to evaluate the efficiency of Enzyme-linked immunosorbent assay (ELISA) assay and monoplex and multiplex real-time reverse-transcription PCR (rRT-PCR) in the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A and B viruses (Flu A and Flu B).
UNASSIGNED: The SARS-CoV-2 -specific IgG and IgM antibodies, as well as, Flu A (H1N1 and H3N2 serotypes) and Flu B virus antibodies were determined by ELISA assay. The one-step qRT-PCR method was used to detect the SARS-CoV-2 in nasopharyngeal swab samples. Furthermore, the presence of Flu A and B viruses was evaluated using probe-based RT-PCR. Simultaneous detection of SARS-CoV-2, Flu A and B viruses was performed by multiplex rRT-PCR assay.
UNASSIGNED: SARS CoV-2 IgM and IgG antibodies were detected in 33.3% and 58.3% of patients, respectively. In contrast, the SARS CoV-2 genome was detected in 50% of patients using the one-step monoplex RT-PCR assay. Flu A serotypes H1N1 and H3N2 were found in 16.7% and 8.3% of patients. Probe-based RT-PCR revealed that 39.3% of patients were positive for the Flu A virus. Multiplex rRT-PCR detect the SARS-CoV-2, Flu A, and Flu B in 50%, 39.3%, and 19% of samples, respectively. The sensitivity and specificity of multiplex rRT-PCR assay in comparison to monoplex RT-PCR were 100% and 55%, respectively. Coinfection with SARS-CoV-2, Flu A, and Flu B viruses was found in 9.5% of patients.
UNASSIGNED: Multiplex rRT-PCR can be used as a repaid, cost-effective and suitable tool for molecular surveillance of SARS-CoV-2 and Flu A/B viruses.
UNASSIGNED: The SARS-CoV-2 -specific IgG and IgM antibodies, as well as, Flu A (H1N1 and H3N2 serotypes) and Flu B virus antibodies were determined by ELISA assay. The one-step qRT-PCR method was used to detect the SARS-CoV-2 in nasopharyngeal swab samples. Furthermore, the presence of Flu A and B viruses was evaluated using probe-based RT-PCR. Simultaneous detection of SARS-CoV-2, Flu A and B viruses was performed by multiplex rRT-PCR assay.
UNASSIGNED: SARS CoV-2 IgM and IgG antibodies were detected in 33.3% and 58.3% of patients, respectively. In contrast, the SARS CoV-2 genome was detected in 50% of patients using the one-step monoplex RT-PCR assay. Flu A serotypes H1N1 and H3N2 were found in 16.7% and 8.3% of patients. Probe-based RT-PCR revealed that 39.3% of patients were positive for the Flu A virus. Multiplex rRT-PCR detect the SARS-CoV-2, Flu A, and Flu B in 50%, 39.3%, and 19% of samples, respectively. The sensitivity and specificity of multiplex rRT-PCR assay in comparison to monoplex RT-PCR were 100% and 55%, respectively. Coinfection with SARS-CoV-2, Flu A, and Flu B viruses was found in 9.5% of patients.
UNASSIGNED: Multiplex rRT-PCR can be used as a repaid, cost-effective and suitable tool for molecular surveillance of SARS-CoV-2 and Flu A/B viruses.
摘要：
■当前的研究旨在评估酶联免疫吸附测定（ELISA）测定以及单重和多重实时逆转录PCR（rRT-PCR）在检测严重急性呼吸综合征冠状病毒中的效率2（SARS-CoV-2）和甲型和乙型流感病毒（甲型流感和乙型流感）。
■SARS-CoV-2特异性IgG和IgM抗体，还有,通过ELISA测定法测定流感A(H1N1和H3N2血清型)和流感病毒B抗体。采用一步qRT-PCR方法检测鼻咽拭子样品中的SARS-CoV-2。此外，使用基于探针的RT-PCR评估甲型和乙型流感病毒的存在。通过多重rRT-PCR方法同时检测SARS-CoV-2，甲型和乙型流感病毒。
■在33.3％和58.3％的患者中检测到SARSCoV-2IgM和IgG抗体，分别。相比之下,使用一步法单重RT-PCR方法在50％的患者中检测到SARSCoV-2基因组。在16.7％和8.3％的患者中发现了甲型流感血清型H1N1和H3N2。基于探针的RT-PCR显示39.3％的患者对甲型流感病毒呈阳性。多重rRT-PCR检测SARS-CoV-2、甲型流感、还有50%的乙型流感,39.3%,和19%的样本，分别。多重rRT-PCR检测的灵敏度和特异度分别为100%和55%,分别。与SARS-CoV-2，流感A，在9.5%的患者中发现了B型流感病毒。
■多重rRT-PCR可以用作偿还，用于SARS-CoV-2和流感病毒A/B的分子监测的具有成本效益和合适的工具。
■SARS-CoV-2特异性IgG和IgM抗体，还有,通过ELISA测定法测定流感A(H1N1和H3N2血清型)和流感病毒B抗体。采用一步qRT-PCR方法检测鼻咽拭子样品中的SARS-CoV-2。此外，使用基于探针的RT-PCR评估甲型和乙型流感病毒的存在。通过多重rRT-PCR方法同时检测SARS-CoV-2，甲型和乙型流感病毒。
■在33.3％和58.3％的患者中检测到SARSCoV-2IgM和IgG抗体，分别。相比之下,使用一步法单重RT-PCR方法在50％的患者中检测到SARSCoV-2基因组。在16.7％和8.3％的患者中发现了甲型流感血清型H1N1和H3N2。基于探针的RT-PCR显示39.3％的患者对甲型流感病毒呈阳性。多重rRT-PCR检测SARS-CoV-2、甲型流感、还有50%的乙型流感,39.3%,和19%的样本，分别。多重rRT-PCR检测的灵敏度和特异度分别为100%和55%,分别。与SARS-CoV-2，流感A，在9.5%的患者中发现了B型流感病毒。
■多重rRT-PCR可以用作偿还，用于SARS-CoV-2和流感病毒A/B的分子监测的具有成本效益和合适的工具。
