Abstract：
Liamocins, a group of high-density glycolipids, are only produced by certain strains of the yeast-like fungi in the genus Aureobasidium. Until now, few studies have focused on the surfactant properties of liamocins produced from the highly diverse tropical strains of Aureobasidium. Therefore, the aims of this research were to screen the liamocin production from tropical strains of Aureobasidium spp. and to characterize their surfactant properties. A total of 41 strains of Thai Aureobasidium spp. were screened for their ability to produce liamocins, and the products were detected using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and thin-layer chromatography. Of those strains, 30 strains of Aureobasidium spp. tested were found to produce liamocins with yields ranging from 0.53 to 10.60 g/l. The nature of all crude liamocins was heterogeneous, with different compositions and ratios depending on the yeast strain. These liamocins exhibited relatively high emulsifying activity against vegetable oils tested, with an emulsification index of around 40-50%; the emulsion stability of some liamocins was up to 30 days. The obtained critical micelle concentration values were varied, with those of liamocins produced from A. pullulans, A. melanogenum and A. thailandense falling in ranges from 7.70 to 119.78, 10.73 to > 1,000, and 68.56 to > 1,000 mg/l, respectively. The emulsification activity of liamocins was higher than that of the analytical grade rhamnolipids. These compounds showed strong surface tension reduction in a sodium chloride concentration range of 2-12% (w/v), pH values between 3 and 7, and temperatures between 4 and 121 °C. This is the first report of liamocins produced by A. thailandense.
摘要：
Liamocins,一组高密度糖脂，仅由金黄色葡萄球菌属的某些酵母样真菌菌株产生。直到现在,很少有研究集中在由高度多样化的热带金黄色葡萄球菌菌株产生的liamocin的表面活性剂特性上。因此,这项研究的目的是从热带金黄色葡萄球菌的热带菌株中筛选liamocin的生产。并表征其表面活性剂的性质。共有41株泰国梭子菌。筛选了它们产生美洲霉素的能力，并使用基质辅助激光解吸/电离飞行时间质谱和薄层色谱法检测产物。在这些菌株中，30株金黄色葡萄球菌。经测试，发现产生的举甲霉素的产量为0.53至10.60g/l。所有粗品的性质都是异质的，根据酵母菌株具有不同的组成和比例。这些金属霉素对所测试的植物油表现出相对较高的乳化活性，乳化指数约为40-50％；一些连霉素的乳化稳定性长达30天。获得的临界胶束浓度值是变化的，与那些从A.pullulans产生的维生素A，黑色素A.和A.thailandense的范围从7.70到119.78，10.73到>1,000和68.56到>1,000mg/l，分别。金属霉素的乳化活性高于分析级鼠李糖脂。这些化合物在2-12％（w/v）的氯化钠浓度范围内表现出强烈的表面张力降低，pH值在3到7之间，温度在4到121°C之间。这是由A.thailandense生产的第一个报告。
