PDF(Pubmed)
Abstract：
Rose flowers (Rosa hybrida L.) are highly perishable and have a limited vase life. This study evaluated the effects of preharvest foliar applications of γ-aminobutyric acid (GABA) and calcium chloride (CaCl2), individually and combined, on antioxidant responses and vase life of cut Jumilia rose flowers. Treatments included foliar sprays of GABA at 0, 20, 40, and 60 mM and CaCl2 at 0, 0.75%, and 1.5%, applied in a factorial design within a completely randomized setup before harvest. Results showed GABA and CaCl2 interaction (especially, 60 mM GABA and 1.5% CaCl2) significantly increased enzymatic antioxidants including superoxide dismutase, catalase, and peroxidase, as well as non-enzymatic antioxidants such as flavonoids, carotenoids, phenolics, and antioxidant activity in petals compared to control. SOD activity in roses, treated with CaCl2 (1.5%) and GABA (60 mM), peaked at 7.86 units. mg-1 protein min-1, showing a nearly 2.93-fold increase over the control (2.68 units. mg-1 protein min-1). A parallel trend was observed for CAT activity. These treatments also reduced petal malondialdehyde content and polyphenol oxidase activity. Protein content and vase life duration increased in all treatments. Plants treated with a combination of GABA (20 mM) and CaCl2 (0.75%), GABA (60 mM) and CaCl2 (1.5%), or GABA (40 mM) individually exhibited the longest vase life duration. The co-application of GABA and CaCl2 improved the antioxidant activity and postharvest quality of cut roses by reducing PPO activity and MDA contents, increasing protein content and prolonging vase life. This treatment is a potential postharvest strategy to improve antioxidant capacity and delay senescence in cut roses.
摘要：
玫瑰花（RosahybridaL.）非常易腐，花瓶寿命有限。这项研究评估了收获前叶面施用γ-氨基丁酸（GABA）和氯化钙（CaCl2）的影响，单独和组合，月季切花的抗氧化反应和花瓶寿命。处理包括0、20、40和60mM的GABA和0、0.75％的CaCl2的叶面喷雾，和1.5%，在收获前的完全随机设置中应用于阶乘设计。结果表明GABA和CaCl2相互作用(特别是，60mMGABA和1.5％CaCl2）显着增加了酶促抗氧化剂，包括超氧化物歧化酶，过氧化氢酶,和过氧化物酶,以及类黄酮等非酶抗氧化剂，类胡萝卜素，酚类物质,与对照相比，花瓣中的抗氧化活性。玫瑰中的SOD活性，用CaCl2(1.5%)和GABA(60mM)处理，峰值为7.86个单位。mg-1蛋白质min-1，显示比对照增加近2.93倍（2.68单位。mg-1蛋白质min-1)。观察到CAT活性的平行趋势。这些处理还降低了花瓣丙二醛含量和多酚氧化酶活性。在所有处理中，蛋白质含量和花瓶寿命都增加了。用GABA（20mM）和CaCl2（0.75％）的组合处理的植物，GABA（60mM）和CaCl2（1.5％），或GABA（40mM）分别表现出最长的花瓶寿命。GABA和CaCl2的共同施用通过降低PPO活性和MDA含量来提高切花玫瑰的抗氧化活性和采后品质。增加蛋白质含量，延长花瓶寿命。这种处理是一种潜在的采后策略，可以提高切花玫瑰的抗氧化能力并延缓衰老。
