PDF(Pubmed)
Abstract：
Aloe barbadensis is a drought-tolerant perennial medicinal plant with both nutritional and cosmetic uses. Drought is one of the main abiotic stresses limiting plant growth and development. However, the use of drought-resistant plants combined with beneficial soil micro-organisms could improve the effectiveness of biological methods to mitigate drought damage. This research aims to evaluate the effects of Funneliformis mosseae (MF), plant growth-promoting rhizobacteria (PGPR) (including Pseudomonas putida and Pantoea agglomerans), and their co-inoculation on the macronutrient status, antioxidant enzyme activities, and other morphophysiological traits of A. barbadensis under four irrigation regimes [25%, 50%, 75% and 100% of water requirement (WR)]. Three harvests were conducted, revealing that inoculation enhanced the survival rate and shoot fresh weight (SFW) compared to the control plants. However, at 25% WR, the SFW was reduced by 43% more than the control. across all harvests, while the PGPR + MF treatment showed increases of more than 19%, 11%, and 17% compared to the control, MF, and PGPR treatments, respectively. The results also showed that A. barbadensis exhibited innate drought tolerance up to a 50% WR level by enhancing physiological defenses, such as antioxidant enzyme activity. Inoculation increased the macronutrient status of the plant at all levels of irrigation regimes especially under severe drought conditions. The highest levels of nitrogen (N) (16.24 mg g-1 DW) and phosphorus (P) (11.29 mg g-1 DW) were observed in the PGPR + MF treatment at 100% WR. The maximum relative water content under MF inoculation and 75% WR (98.24%) (98.24%) was reached. PGPR + MF treatment alleviated drought-induced osmotic stress, as indicated by reduced antioxidant enzyme activities and electrolyte leakage. However, P. putida and P. agglomerans strains alone or in combination with F. mosseae increased plant yield, macronutrient uptake and antioxidant enzyme activity. This study underscores the potential of these PGPR and MF strains as invaluable biological tools for the cultivation of A. barbadensis in regions with severe drought stress.
摘要：
库拉索芦荟是一种耐旱的多年生药用植物，具有营养和化妆品用途。干旱是限制植物生长发育的主要非生物胁迫之一。然而,抗旱植物与有益土壤微生物的结合使用可以提高生物方法减轻干旱损害的有效性。本研究旨在评估真菌真菌（MF）的影响，植物生长促进根际细菌（PGPR）（包括恶臭假单胞菌和泛菌），以及它们对大量营养素状态的共同接种，抗氧化酶活性，在四种灌溉制度下[25%，50%,75%和100%的需水量(WR)]。进行了三次收获，与对照植物相比，接种提高了存活率和芽鲜重（SFW）。然而,在25%WR，SFW比对照组减少了43%。在所有收成中，而PGPR+MF治疗显示增加超过19%，11%,与对照组相比，为17%，MF,和PGPR治疗，分别。结果还表明，通过增强生理防御能力，巴巴多斯表现出高达50％WR水平的先天耐旱性，如抗氧化酶活性。在所有灌溉制度下，尤其是在严重干旱条件下，接种都增加了植物的常量营养素状况。在100％WR的PGPRMF处理中观察到最高水平的氮（N）（16.24mgg-1DW）和磷（P）（11.29mgg-1DW）。在MF接种和75％WR（98.24％）（98.24％）下达到最大相对含水量。PGPR+MF处理缓解了干旱诱导的渗透胁迫，抗氧化酶活性降低和电解质渗漏表明。然而,P.putida和P.agglumans菌株单独或与F.mosseae增加植物产量，大量营养素吸收和抗氧化酶活性。这项研究强调了这些PGPR和MF菌株作为在严重干旱胁迫地区种植A.barbadensis的宝贵生物工具的潜力。
