Abstract：
Monitoring wildlife health is essential for understanding global disease patterns, particularly as vector-borne infections extend the geographic ranges and thereby hosts due to environmental shifts. Anaplasma marginale, primarily impacting cattle, has economic implications and has been found in diverse hosts, yet its presence in capybaras (Hydrochoerus hydrochaeris), influential in tick-borne pathogen spread, lacks comprehensive understanding. From 2015 to 2022, 14 capybaras were surveyed across two different areas of northeastern Argentina. In 1 of 14 (7%) capybaras, the presence of A. marginale was confirmed through the amplification of specific genes, msp5 and msp1β. In addition, A. marginale DNA was detected in the capybara\'s blood sample through quantitative PCR, with a cycle threshold value of 30.81 (800 copies per reaction). Amplification of a fragment of the msp1α gene revealed PCR products of three different sizes, suggesting the presence of at least three coinfecting A. marginale variants in the capybara host. This study suggests that capybaras are wild hosts for A. marginale in the Ibera Wetlands in Argentina, potentially influencing the infection dynamics of both domestic and wild species. This finding highlights the necessity for thorough studies on the role of capybaras in disease dynamics, crucial for understanding wildlife health and the spread of disease.
摘要：
监测野生动物健康对于了解全球疾病模式至关重要，特别是由于媒介传播的感染扩展了地理范围，从而由于环境变化而成为宿主。边缘无性体，主要影响牛，具有经济意义，并在不同的宿主中发现，然而，它存在于水蛭(Hydrochoerushydrochaeris)中，影响蜱传播的病原体传播，缺乏全面的了解。从2015年到2022年，在阿根廷东北部的两个不同地区对14只水豚进行了调查。在14个（7％）的鱼中，通过特定基因的扩增证实了边缘A.marginale的存在，msp5和msp1β。此外,A.通过定量PCR在水痘的血液样本中检测到边缘DNA，循环阈值为30.81（每个反应800个拷贝）。msp1α基因片段的扩增显示了三种不同大小的PCR产物，这表明水豚宿主中至少存在三种共感染边缘A.margenale变体。这项研究表明，在阿根廷的伊贝拉湿地中，水豚是A.marginale的野生寄主，潜在影响家养和野生物种的感染动态。这一发现强调了彻底研究水蛭在疾病动力学中的作用的必要性。对于了解野生动物健康和疾病传播至关重要。
