Abstract：
The nucleus accumbens (NAc) and the anterior limb of internal capsule (ALIC) are effective targets for treating addiction using deep brain stimulation (DBS). However, there have been no reports on the electrophysiological characteristics of addiction nuclei at the single-cell level in humans. This study aimed to investigate the electrical activity characteristics of neurons in the NAc and ALIC using Microelectrode Recording (MER) during DBS surgery in patients with addiction, and six patients with addiction were included (five with heroin addiction and one with alcohol addiction). The microelectrode recording trajectories were reconstructed and recording sites at different depths were determined by merging the pre- and post-operative images in the FrameLink system. The results showed that among the 256 neurons, 204 (80 %) were burst neurons. NAc neurons accounted for the majority (57 %), and the mean firing rate (MFR) was the highest (1.94 Hz). ALIC neurons accounted for the least (14 %), and MFR was the lowest (0.44 Hz). MFR increased after entering the NAc and decreased after exiting the ALIC. In the patients with addiction treated using DBS, the single-cell level electrophysiological characteristics of the different nuclei were found to be distinct along the surgical trajectory.
摘要：
伏隔核（NAc）和内囊前肢（ALIC）是使用深部脑刺激（DBS）治疗成瘾的有效靶标。然而,目前还没有关于人类单细胞水平上成瘾核的电生理特性的报道。本研究旨在探讨成瘾患者DBS手术期间使用微电极记录（MER）的NAc和ALIC中神经元的电活动特征，包括6名成瘾患者(5名海洛因成瘾和1名酒精成瘾)。通过合并FrameLink系统中的术前和术后图像，重建了微电极记录轨迹，并确定了不同深度的记录位点。结果表明，在256个神经元中，204(80%)为爆发神经元。NAc神经元占多数（57%），平均放电率（MFR）最高（1.94Hz）。ALIC神经元占最少（14%），MFR最低（0.44Hz）。MFR在进入NAc后增加,在离开ALIC后降低。在使用DBS治疗的成瘾患者中，发现不同细胞核的单细胞水平的电生理特征在手术轨迹上是不同的。
