Abstract：
Liquid-transmission electron microscopy (liquid-TEM) provides exciting potential for capturing mineralization events at biomaterial interfaces, though it is largely unexplored. To address this, we established a unique approach to visualize calcium phosphate (CaP)-titanium (Ti) interfacial mineralization events by combining the nanofabrication of Ti lamellae by focused ion beam with in situ liquid-TEM. Multiphasic CaP particles were observed to nucleate, adhere, and form different assemblies onto and adjacent to Ti lamellae. Here, we discuss new approaches for exploring the interaction between biomaterials and liquids at the nanoscale. Driving this technology is crucial for understanding and controlling biomineralization to improve implant osseointegration and direct new pathways for mineralized tissue disease treatment in the future.
摘要：
液体透射电子显微镜（液体TEM）为捕获生物材料界面的矿化事件提供了令人兴奋的潜力。虽然它在很大程度上是未经探索的。为了解决这个问题，我们建立了一种独特的方法来可视化磷酸钙（CaP）-钛（Ti）界面矿化事件通过结合Ti薄片的纳米加工聚焦离子束与原位液体TEM。观察到多相CaP颗粒成核，坚持,并在Ti薄片上和附近形成不同的组件。这里,我们讨论了探索纳米尺度上生物材料与液体相互作用的新方法。推动这项技术对于理解和控制生物矿化以改善植入物骨整合和指导未来矿化组织疾病治疗的新途径至关重要。
